68 



Proceedwgs of the Royal Irish Academy. 



Both organisms agree in their tinctorial characters, and generally 

 in their microscopic appearances. 



The organism I have utilized was isolated by me direct from the 

 liver of a turkey, and has been sub-cultured over a period of two 

 years. It grows readily and well upon the usual media, glycerine 

 blood-serum, glycerine agar, and glycerine potato. It also grows in, 

 and on, bouillon containing 5 per cent, glycerine. It has maintained 

 its pathogenic properties constantly, and apparently without attenua- 

 tion. 



As I was desirous of ascertaining the pathogenicity of the 

 organism I had isolated for bovines, and particularly as I was un- 

 acquainted with any recent experiments made with cultures of the 

 avian bacillus upon bovines, I determined to utilize two cattle which 

 I had in my possession. They were submitted to the usual tuberculin 

 test, with the object of determining their freedom from tuberculosis. 

 There was no reaction, and consequently they were declared free from 

 tuberculosis. The animals were a heifer and a young bull, both of 

 the same age, approaching two years. The virus I employed was a 

 culture in bouillon (glycerinated) of the third generation. The heifer 

 received 5 c.c. of the bouillon into the auricular vein, on September 4, 

 1906. The infection was first attempted into a vein of the left ear; 

 but, the animal moving when the injection was about to be made, 

 the needle left the vein, and a small quantity of the fluid escaped 

 into the tissues. The injection was then made into a vein of the 

 right ear, the quantity being made up to 5 c.c. A fortnight later a 

 small swelling was noticed at the root of the left ear where the first 

 injection had been attempted; but it remained hard and did not 

 suppurate. 



On September 25 the animal was noticed to be coughing, and the 

 respirations were slightly increased in frequency. The temperature 

 was 106° F. On succeeding days, until death, the temperature was 

 taken morning and evening. The subject continued to cough. The 

 respirations were hurried, the eyes sank, the animal lost condition, 

 and symptoms of pneumonia supervened. The temperatures recorded 

 are of interest, and I append them : — 







A.M. 



P.M. 



September 26 



104-4 



104-2 



j> 



27 



104-6 



106-0 



>> 



28 



104-0 



105-6 



)j 



29 



104-0 



105-8 



>) 



30 



104-8 



106-2 



