582 



GABRIELE KASS-SIMON 



and at 50% of their total length and the Time of Tentacle Differentiation (TDT), 

 the Final Tentacle Number (FTN), the Initial Tentacle Number (ITN), the 

 différence between the two (FTN — ITN), and the Tentacle Régénération Factor 

 (TRF), as defined in Section II, compared. 

 TRF 

 300 



200 



100 



o BUDDING ANIMALS 

 • CONTROLS 



AXIAL 



JBT 



75°o 



50 q o 



LEVEL 



Fie. 7. 



MeanTRF's for non-budding and budding week-old animais at three axial levels. 

 The values for budding animais differ significantly from one another. Those for 

 non-budding animais do not. The différence at each level between budding and 

 non-budding animais is not significant. (TRF=Tentacle Régénération Factor, 

 JBT=Just Below Tentacles. The Connecting lines are intended only to show 

 that the déclines are not steady). 



2. Comparison of week-old budding and non-budding animais 

 eut at 50% 



In a second experiment week-old budding and non-budding animais from 

 clone 3a ? were fed 6 units of food each three days after séparation from their 

 parent animais and transected at 50% of their total length. Their régénération 

 performance was evaluated as above. 



Ail buds were in early or middle stages of development, as defined in Sec- 

 tion IV.d. (p. 585). 



(c) Results 



1 . Régénération at various levels of budding and non-budding 

 " Standard " animais 



The data are given in Table 7 and Figure 8. The différences in TDT's were not 

 significant although there is an apparent delay for budding animais eut at 50%. 



