590 GABRIELE KASS-SIMON 



Group IV. Day-old animais given (1) 6 units of food, (2) 1 unit of food, (3) no food. 





TDT 



N 



FTN 



N 



ITN 



N 



FTN— ITN 



N 



TRF 



N 



BDT 



N 



(1) 



-> "\ f\r\ i i a r\ 



32.00 zk 1.40 

 6 



C Ol 1 A CtO 



D.oi ±: O.^o 

 6 



1 /C/C 1 1 AC 



2.66 zh 105 

 6 



3.33 — 1.54 

 6 



253 i 143 

 6 



41.30 ± 3.30 

 6 



(2) 



40.00 ± 3.80 

 10 



5.10 ± 0.57 

 10 



2.40 4- 1.02 

 10 



2.70-4- 1.01 

 10 



215 ± 156 

 10 



41.00 ± 0.00 

 10 



(3) 



38.00 ± 5.10 

 8 



5.30+ 1.38 

 8 



2.75 ± 0.89 

 8 



2.25 ± 1.50 

 8 



199 ± 90 



8 



43.40 ± 7.8 



7 



The Time of Basai Plate Differentiation was also found not to vary signifi- 

 cantly, both in inter and intra group comparison, indicating that here, at least, 

 basai plate régénération was not influencée! by différences in âge and feeding 

 conditions. 



(d) Discussion 



Although animais in both experiments were treated comparably, the results 

 do not completely coincide (Compare Table 9 with Table 10, Experiment 1 with 

 Experiment 2, Group IV), and it is conjectured that différences in pretreatment of 

 mother animais resuit in varying physiological states of the expérimental animais. 

 (For example, it appears that animais stemming from mothers which had been fed 

 until séparation at the end of a given five day period contained more protein than 

 those splitting off at the beginning of that period, although the animais themselves 

 were equally fed (Kass, unpublished data)). It is entirely possible, therefore, that 

 in the second experiment, since one clone was used continuously, that at the onset I 

 of the experiment, a maximum of reserves was already présent and that additional : 

 food, on the one hand, and deprivation, on the other, could not significantly affect 

 the process in question which was already more than adequately supplied. Further, j 

 animais were transected at 50 %, which, as already stated (Section III. d.), represents i 

 that area adjacent to and in part including the budding zone. Two animais from ; 

 Group I (not included in the data) performed buds before opération and in the I 

 preceding section on Budding, ail animais fed three days after séparation made I 

 buds before transection on the seventh day, indicating, that perhaps, something 

 approaching a maximum had indeed been reached and that additional feeding 

 rather than promulgating régénération in the budding région, leads to a change 

 in that région which, as has been suggested, is, at least partially, antithetical to 

 the regenerative process. In this regard, Burnett (1961b) has found that there is 

 an accumulation of pigment in the budding zone immediately before buds become 



