856 



N. F. GOLDSMITH, H. HUGGEL AND H. K. URY 



0.14 + 0.36 mEq/1. In expt. 4, normal females, 158 g., 2 punctures only, complète 

 Altromin diet, AMg was 0.34 mEq/1, ACa 0.11 mEq/1. Computed data for 

 AMg, ACa, AK and AP0 4 in normal rats on various diets after 2-4 punctures 

 are given in Table 2. 



AMg, AK, ACa in Enovid-injected animais were substantially less than the 

 values in oil-injected rats (Table 2, Girard diet, expt. 6). On magnesium-poor 

 Altromin diet, AMg for Enovid-injected rats was one-third the values for control 

 rats (Table 2, expt. 7). The observed magnésium increase was signiflcantly less in 

 the Enovid-injected groups p < .005 (Table 3); body weights, etherization and 

 withdrawal times and sample volumes were not signiflcantly différent between 

 groups (t test). 



DISCUSSION 



During blood loss by cardiac puncture, hydrogen ion accumulated in the 

 blood (measured as a fall in blood pH) by several possible mechanisms: dépression 

 of respiration during anesthesia; interruption of respiratory exchange after 

 thoracotomy; decreased blood flow, stasis and diminished buffer capacity after 

 blood loss; and continued hydrogen production from cellular metabolism. Fig. 4 

 compares sérum magnésium increases after hemorrhage to in vitro studies of 



.80 



cr 

 UJ 



E 



2 



60 



40 



.20 



7.6 



7.4 7.2 

 pH 



7.0 



...a 



6.8 



Fig. 4. 



As pH dccrcascd, frcc bivalent cations increased, hemorrhage in rats compared with calcium 



binding studies in vitro, o o rat hemorrhage (expt. 2) Mg + + ; o o 2% sérum 



albumin, Ca, + ♦ Carr (1953); A A rat microsomes, Ca, + + Carvalho (1963). 



