860 



N. F. GOLDSMITH, H. HUGGEL AND H. K. URY 



déficient diet, it is likely that less magnésium was bound and available for release 

 during hemorrhage. Correspondingly, lowered concentrations of sérum magnésium 

 in women using contraceptive drugs may resuit in decreased amounts of magnésium 

 bound to intravascular membranes and proteins. 



Enovid-injected rats on the Girard diet seemed to have less sérum magnésium, 

 calcium and potassium available compared to oil-injected controls; phosphorus 

 concentrations were not différent. Since urinary and fecal cations were also lower 

 after Enovid injection (unpublished data), while osseous magnésium increased 

 (Goldsmith and Baumberger, 1967), the sérum changes could reflect a gênerai 

 accumulation of cation at the bone. 



In the oil-injected rats on magnesium-poor Altromin diet, ACa was 

 0.07 mEq/1, one-third or less than control and Enovid-injected groups. On Girard 

 diet, oil-injected rats after 3-4 daily injections apparently had a greater calcium 

 shift with hemorrhage than control and Enovid-injected groups. However, after 

 6-15 daily injections, oil-injected groups appeared to have less calcium in cardiac 

 muscle, although the number of animais (4 per group) was too small to détermine 

 significance: control 9.2 + 2.5, oil-injected, 8.2 ± 2.2, Enovid-injected 9.4 ± 

 2.3 mEq/gm muscle. After 26-28 injections, oil-injected animais had signifkantly 

 less osseous calcium than controls (Goldsmith and Baumberger, 1967). During 

 15 days of observation, daily fecal and urinary calcium, magnésium and potassium 

 concentrations in the oil-injected groups were not significantly différent from 

 controls. We have no explanation for the apparent decrease in calcium binding 

 after prolonged corn oil injection, but similar observations have been made in rats 

 fed a diet high in corn oil and with Ca/Mg = 7 (Hellerstein et al, 1960). 



SUMMARY 



To test for sérum ion availability, Sprague-Dawley rats were bled by repeated 

 punctures of the right ventricle. Total blood volume and the percent hemorrhage 

 of each blood sample were calculated from the équation of Fernandez, Rettori and 

 Mejia. Approximately 40 percent of the total blood volume was withdrawn in 

 less than 100 seconds. Comparing successive blood samples obtained by cardiac 

 puncture, pH and glass clotting time were diminished, clot elasticity was augmented, 

 and sérum magnésium, calcium, potassium and phosphate concentrations were 

 increased. 



To compare groups of animais, average values for sérum ions at observed 

 hemorrhage points were used to construct an average slope. The slope was then 

 extended to a référence interval between 20 and 40 percent hemorrhage. In normal 

 rats the average increase in sérum magnésium concentrations between 20 and 40 per- 

 cent hemorrhage was 0.32 mEq/1. With a magnesium-poor diet, the magnésium 



