370 



THE AMERICAN NATURALIST 



[Vol. LV 



tilization by oeeupyino- the spermophilo side-chain ; yet, "anti- 

 fertiliziii" also prevents ant()-parthenop:enesis (Woodward). 

 This, we miglit suppose, would intlicate a relationship between 

 the two side-chains, such that removal from tlu^ reaction system 

 of the one also results in tlie removal of the other. This state 

 of afi'airs is actually realized under certain conditions. For ex- 

 ample. 1 have been able to isolate from charcoal materials with 

 atrglutinating and activating properties. However, if we infer 

 from these observations, a sinjrlc body, the reaction of "anti- 

 fertilizin" with fertilizin can hardly serve as evidence that the 

 latter has two side-chains. 



IV 



In 1918, Miss Woodward* reported experiments tliat bear in- 

 timately on the pi'ohhMii. I'.y s;!tu ra1 iuo' Arhuria s.nMvtion with 

 (Nn,),SO, ^hr sr,Mir,Ml a uhit.. flMcruh-Mt pivripihitr wliich after 



She called tlii. p'n cipilatc ii--luliniii. 



An initiatory ;,.^hit. akn, u,is pi'^vipitat..!. was added 



to the seerelion an-l .-ift.-r nM.K.Md of tlh- M-a-^alK. tlir primary 

 deposit was treated with V 10 II("1. As vomi iti.' a.-id had 

 b<'<-n freed from BaCl,, acetone was used in ex— \,nu- dnuu 

 a second precipitate, heavy and flocciilent. Tins, alt' !■ puri!ii-a- 

 tion with absolute alcohol and ether, dried as a whifr powder, 

 soluble in both sea-water and distilled. 



This second precipitate had marked partheno<2enetic effects, 

 but no power to ag*rlutinate sperm. Miss Woodward called the 



