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THE AMERICAN NATURALIST [Vol. LIV 



(1) Sat. aq. sol. methylene blue. 



(2) Sat. aq. sol. methyl green. 



(3) Sat. aq. sol. gentian violet. 



(4) Sat. aq. sol. safranin. 



B. For staining after killing, or for killing with the stain itself. 

 (Make up a quantity of the stain, and dilute with water for 

 desired color.) 



(1) Sat. alcoholic sol. methylene blue. 



(2) Sat. alcoholic sol. methyl green. 



(3) Sat. alcoholic sol. gentian violet. 



(4) Sat. alcoholic sol. safranin. 



(5) Sat. alcoholic sol. iodine, with 3 per cent, potassium 



iodide. This gives excellent results. It is a very pow- 

 erful stain, and must be used in weak solutions. 



