88 



Messrs. Jones and Dupre on a Substance^ [Apr. 12, 



Professor Bonders has carefully investigated the time in which atropine 

 and Old Calahar bean begin and cease to act on the iris in man. 



A solution of atropine dropped upon the cornea began to act in fifteen 

 minuteSj and attained its maximum in from twenty to twenty -five minutes. 

 In forty-two hours the pupil was rather smaller; and even after thirteen days 

 the pupil had not returned to its natural size. 



The solution of Calabar bean began to act in from five to ten minutes. 

 It attained its maximum in from thirty to forty minutes. At the end of 

 three hours it began to diminish, and its effect disappeared entirely in from 

 two to four days. 



After continued applications of belladonna to the eye of a rabbit, it was 

 thoroughly washed by a full current of water. The aqueous humour was 

 then evacuated and brought into contact for a long time with the eye of a 

 dog (De Graefe injected the aqueous humour into the anterior chamber) ; 

 then a notable dilatation of the pupil was observed. As one part in 

 120,000 of water acts very energetically, the quantity must be very little 

 that produced the dilatation of the pupil. 



When belladonna used internally produces the dilatation, the aqueous 

 humour which is taken from the anterior chamber is inactive. 



Thirdly. The fluorescence that naturally occurs in different parts of the 

 human body when no quinine had been taken before death was determined, 

 in order that the effect of quinine on the fluorescence in the same parts 

 might be estimated : — 



The different parts were dried in a water- bath, and equal quantities of 

 the dried substance were taken, amounting to 0*6 grain, that being the 

 weight of the dry lens. The same method' of extraction was followed. 



The solution was in all cases made up to twenty-five grains. 



Extract from per 100 litres. 



Cartilage fluoresced one 32nd of a grain of quinine to a litre . . =3*1 

 Nerves fluoresced a little more than one 64th of a grain of 



quinine to a litre =1'6 



Liver fluoresced a little more than one 64th of a grain of 



quinine to a litre =1*6 



Kidney fluoresced a little more than one 64th of a grain of 



quinine to a litre =1*6 



Lens fluoresced a little less than one 64th of a grain of quinine 



to a litre =1*6 



Lungs fluoresced one 128th to one 64th of a grain of quinine 



to a litre =0-8 to 1*6 



Muscle fluoresced a little more than one 128th of a grain of 



quinine to a litre. =0*8 



Spleen fluoresced one 128th of a grain of quinine to a Htre. . =0*8 



In another patient, who died after a surgical operation, the same quan- 

 tity of substance, treated in exactly the ^ame way, gave — 



