The tubes were iiiciil)at(Ml from twenty-four to thirty-six hours 



white, raised o-n.wili wlicrr tlu' mr.liuin was iiioeulated. The 

 series of inoculations wen' made a week apart from May 1 to 

 about September 1, 15)14. The work of 191'}, while similar in 

 nature, was not so extensive^ as that of 1914. The experiments 

 thus extended over the period of tlie year when the trees are 

 making their most rapid growth, and should be in their most 

 susceptible condition for infection. The trees were well cared 

 for and made rapid growth during the period the experiments 

 were in progress, and hence were in favorable condition for the 

 development of the disease. No effort was made to protect in 

 any way the punctures, as the use of wax or other covering stim- 

 ulates callus formation which could easily be confused with the 

 beginning stages of a young gall or with one that has not ma- 

 tured rapidly, as is often the case on inoculated trees showing 

 resistance. 



The genus Prumis gives a wide range for investigation be- 

 cause of the large number of species and varieties. The follow- 

 ing are the species thus far tested by artificial inoculations: 



