FURTHER OBSERVATIONS ON THE EFFECTS 

 OF ALCOHOL ON WHITE MICE^ 



L. B. NICE 



a former paper ('11) it was found that white mice 

 were not markedly affected when given alcohol in their 

 food. Since this paper appeared Stockard ( '12, '13, '16) 

 has brought forth some striking and conclusive results 

 demonstrating that guinea pigs are very sensitive to 

 alcohol and decidedly injured by it. He administered the 

 alcohol to his animals through the lungs by placing them 

 in a tank containing alcohol at the bottom so that they had 

 to inhale the fumes. His work raised the question as to 

 whether similar results might be obtained with white mice 

 by using the same method. Therefore it was decided to 

 repeat my experiments, using the inhalation method. 



For these experiments white mice eleven weeks old 

 were obtained. They were all from one strain inbred to 

 the fourth generation, two entirely distinct strains having 

 been united to form this strain.. They were divided into 

 four lines, viz., a control line, a double alcohol line, that is, 

 both males and females were subjected to alcohol, a female 

 alcohol line and a male alcohol line. There were three 

 cages in each line, each cage contained two females and 

 one male, thus making six females and three males in each 

 line. Two cages were made up of second generation al- 

 coholized mice; both males were from the male alcohol 

 line, two females from"the same line and one female from 

 the female alcohol line. The same cages were used as in 

 my former experiments. They were made of 8-mesh wire 

 and were 6 inches wide, 6 inches deep and 12 inches long. 



The mice were kept in a laboratory room heated by 

 steam. It was attempted to keep the room at 

 temperature, but fluctuations occurred. 



1 From the Laboratory of Physiology in the T^iiversity 

 596 



