No. 518] GENETIC AL STUDIES ON (ENOTHERA 109 



the characteristic habit and foliage of gigas; the large 

 flowers and stout buds, ovaries and seed capsules, were 

 also gigas-like. Some eighty attempts to obtain seed by 

 guarded self pollination gave complete failures, which 

 were the more interesting because ovaries presumably 

 pollinated from neighboring Oenotheras by the numerous 

 insect visitors matured large fruits and quantities of 

 seeds; the failure to self -pollinate was thus apparently 

 not due to abnormalities of the ovules. De Vries 1 re- 

 ports that he has obtained fertile seed of this hybrid as 

 well as of the reciprocal cross. There was a large pro- 

 portion of abortive pollen, perhaps 80-90 per cent., but 

 the remaining grains, as regards outward appearances, 

 seemed normal. These latter consisted of a mixture of 

 3-lobed and 4-lobed grains in various proportions for dif- 

 ferent plants, the 3-lobed preponderating. Four-lobed 

 pollen grains, as reported by Miss Lutz, 2 are character- 

 istic of gigas. 



2. muricata X gigas. The gigas parent furnishing 

 the pollen of this cross was the same as in the preceding 

 culture; the muricata parent was a wild plant from 

 Woods Hole transplanted as a rosette to the garden of 

 1908. Hybrids of these were among the most interesting 

 of the cultures of 1909. The well-developed rosettes 

 were strikingly intermediate between those of the two 

 parents, but fell clearly into two groups. Of the twelve 

 plants in the culture six rosettes had leaves muricata-\ike 

 in form but of a larger size than is typical of this species, 

 and six rosettes had leaves broader than the above and 

 conspicuously crinkled like the gigas parent. 



The mature plants exhibited a similar difference; the 

 first six had a habit and foliage that were muricataAike, 

 the leaves, however, being longer and 2-3 times as broad ; 

 the other six plants presented a much stockier gigas-like 

 habit with a stouter main stem and with leaves, which on 

 the lower portion of the stem were strongly crinkled as in 

 gigas. The flowers, inflorescences and capsules were 



*De Vries, H. ('085), "Bastarde von (Enothera gigas," Ber. deut. hot. 

 Gesell., XXVIa, 754, 1908. 



*Lutz Anne M.. "Notes on the First Generation Hybrid of (Enothera 



