238 Cincinnati Society of Natural History. 



Glucose Gelatin. — Growth throughout tube but more abun- 

 dant at surface. No gas. 



Gelatin Slant. — Rather slight growth at first, which in- 

 creases slowly. Quite irregular and shiny, but becomes 

 wrinkled with age. No liquefaction. Sulphur-yellow. 



Agar Slant. — Quite abundant sulphur- yellow growth. 

 Regular, moist and shiny. Spreads slowly. 



Bouillon. — Uniform cloudiness throughout medium. No 

 surface growth. Yellowish precipitate. 



Glucose Bouillon. — No gas. Uniform cloudiness. Pre- 

 cipitate. 



Milk. — No apparent change. Sulphur-yellow growth on 

 sides of tube at the surface. 



Potato. — Slight sulphur-yellow growth which does not in- 

 crease and soon dries up. If kept in moist chamber, abun- 

 dant growth takes place producing moist, shiny, raised ridges. 

 Potato is not changed. 



A 3 , 



Morphology. — Small oval bacillus. Non-motile. Single, but 

 may form short irregular chains. Tendency to involution 

 forms in old potato cultures. Stains readily. Size, .75 p- 

 wide and 1.5 <>■ long. Rounded ends. 



Gelatin Plate. — A small, round, irregular and white colony, 

 which increases considerably in size with age. After some 

 time very slight liquefaction of the medium. 



Gelatin Stab. — Abundant growth along track. Most abun- 

 dant toward surface. Grey color. After ten days a slight 

 pit forms which increases slowly. 



Glucose Gelatin. — Slight growth. No gas production. 



Gelatin Slant. — At first slight growth, but this becomes 

 quite abundant with time. White, slightly irregular and 

 shiny. Slight liquefaction after ten days. 



Agar Slant. — Very abundant, white, spreading, moist and 

 shiny growth. Opaque. 



Bouillon. — Heavy and uniform cloudiness. Abundant 

 white precipitate. 



Glucose Bouillon. — No gas. 



Milk. — No change at either room temperature or blood heat. 

 Potato. — Abundant growth with age. Irregular outline, 

 surface irregular and warty. White, with creamy tint. 



28 



