9 



and perfectly healthy otherwise, had a spot about 2 cm. in diam- 

 eter. This part was a delicate yellow mottled with red, and ap- 

 parently did not change in size or color in the two months it was 

 Ob served , 



The next stage (No. 2) was slightly darker than the 

 first, and from this a culture of Alternaria was readily obtained, 

 although the mycelium was not abundant in the tissue. The fruit 

 in this case was yellow, and the spot slightly darker, mottled 

 with both red and brown. The shape and texture appeared the 

 same as in the rest of the fruit. 



The same characteristics as in the first two appeared 

 also in No. 3, but in the center was a slightly sunken brown spot 

 with a distinct border, and radiating around this was the yellow 

 tissue, mottled with red. The brown spot was irregular in shape, 

 (See Plate II, No. Ill) shiny, hard, and from 2 to 4 cm. in diam- 

 eter. 



In the more advanced stages the red color usually in- 

 creased. There was still the yellow color mottled or striped 

 with red, or the green blushed with red and the irregular brown 

 spots in the center of the rounded more developed ones. In some 

 cases there was only a narrow border of red outside the brown. 

 (Plate II, No. IV). The texture of these spots was decidedly 

 leathery and hard and in many cases the surface was slightly de- 

 pressed, o^ten the roundness of the apple would be changed, 

 showing that there had been a stoppage in the growth of the cells 

 on that side. It is possible that these irregular spots co- 



