Trypanosomes found in Wild G. morsitans and Wild Game. 39 



along the dusty tracks made by the antelope and around the pools where 

 they drink. 



Nandumba's village (14° 40' S. lat., 35° 10' E. long.), on the banks of the 

 Shire Paver, was selected as the locality for the camp in which to carry out 

 •experiments of feeding flies on healthy dogs and goats, monkeys being 

 unobtainable. The experiments were carried out between the dates of 

 June 19 and July 25, 1913. 



Methods Employed. 



The method employed in the feeding experiments was the same as 

 described in a previous paper in the ' Proceedings,'* except that monkeys 

 were unobtainable, and the flies were fed only twice on each animal. 



All infected animals were subsequently taken to Kasu, the usual pre- 

 cautions being taken to prevent re-infection on the way, and the trypano- 

 somes found in them were compared with the species and strains of 

 trypanosomes obtained from human beings, various animals, and the flies in 

 the Proclaimed Sleeping Sickness Area. Special attention and study were 

 devoted to the comparison of the strain of the trypanosome causing disease 

 in man in Nyasaland — Trypanosoma brucei vel rhoclesiense — from Nandumba's, 

 with strains obtained from human beings, various animals, and the tsetse flies 

 in the Proclaimed Area. 



The following Table gives in the first column the date the tsetse flies were 

 first fed on the experimental animals, the second column the number of flies 

 fed, and the signs plus and minus show the result of feeding the flies on the 

 dog and goat. 



Table I. — Infectivity of Wild Glossina morsitans in the Liwonde District. 







Dog. 



G-oat. 





No. of 



















Date. 



flies 

 fed. 



a 



q) q, 



s 







a 



£ .5 



•mn.it 



S8 









.5 ^ 



O 



St 







I* 



Si 



T. brut 

 rhodesn 



T. feet 



s 



T. cap'i 



1913. 



June 19 



73 



+ 











+ 



+ 



+ 



„ 26 



150 



+ 



+ 









+ 





+ 



July 2 



100 



+ 











+ 





+ 



„ 4 



450 



+ 











+ 





+ 



„ 8 



650 



+ 



+ 













+ 



„ 15 ; 



650 



+ 











+ 







» 22 



500 





+ 









+ 

























* £ Eoy. Soc. Soc.,' B, vol. 86, pp. 422 and 423 (1913). 



