40 Trypanosomes found in Wild G. morsitans and Wild Game. 



It will be seen that the " fly " in the Upper Shire district carries the same 

 four species of trypanosomes as those found at Kasu in flies from the 

 Proclaimed Sleeping Sickness Area : T. brucei vel rhodesiense, T. pecorum, 

 T. simice, and T. caprce. 



Here, in a series of seven experiments, all the animals on which the flies 

 were fed developed trypanosome disease. In six experiments the flies 

 infected the dogs with T. brucei vel rhodesiense ; in the second and fifth 

 there was a double infection with T. pecorum ; and in the seventh an 

 infection with T. pecorum alone. None of the goats were infected with 

 T. brucei. Six goats were infected with T. pecorum, one with T. simice, and 

 five with T. caprce. It will be noticed that the smallest batch of flies used, 

 a batch of 73, infected the dog with T. brucei, and the goat with T. pecorum, 

 T. simice, and T. caprce. 



Examination of the Blood of Wild Animals in the Liwonde 



District. 



Whenever wild animals were killed their blood was examined for trypano- 

 somes, which were identified by the microscope in, stained films of the blood. 

 The following Table gives the results : — 



Table II. 





Species of trypanosomes found. 



Animal. 















T. brucei vel 

 rhodesiense. 



T. pecorum. 



T. simiie. 



T. caprx. 



T. ingens. 



































+ 









+ 

 + 

















+ 





















+ 











+ 



+ 







+ 











+ 



_ 



This Table, even in so small a series of animals examined, indicates that 

 T. pecorum occurs frequently in the wild game, such as the impala and 



