Carrier of Trypanosome Disease in Nyasaland. 31 

 (b) Details of the Three Experiments. 



Table XII. 



Expt. 



Day of 

 expt. 



Procedure. 



Eernarks. 



2071 



1-8 

 9-10 

 11-12 



Flies fed on infected Goat 1912. 

 Starved. 



Fed on clean Goat 2103. 



11 flies used ; 1 infected fly found. 

 Only 3 dissected. 



2199 



1-8 



9 



10-28 

 29-30 

 31-50 



Flies fed on infected Goat 1912. 

 Starved. 



Fed on clean Goat 2212. 

 Starved. 



Fed on clean Goat 2245. 



60 flies used ; 1 infected fly found. 

 25 dissected. Goat 2212 showed 

 trypanosomes on the 27th day. 

 Goat 2245 negative. 



2277 



1-5 



6-7 



8-32 



33-34 



35-49 



Flies fed on infected Goat 2220. 

 Starved. 



Fed on clean Goat 2287. 

 Starved. 



Fed on clean Goat 2362. 



50 flies used. No flies dissected. 

 Goat 2387 showed trypanosomes on 

 the 31st day, and Goat' 2362 on the 

 49th day. 



(c) Result of the Dissection of the Two Infected Flies. 



Table XIII. 



Expt. 



Time, days. 



Proboscis. 



Alimentary 

 tract. 



Salivary 

 glands. 



Labial cavity. 



Hypopharynx. 



2071 

 2199 



9 

 45 



+ 



+ + 



+ + 



+ + + 





In Experiment 2071 there is evidently a development of T. caprce in the 

 tly found infected. The intestine and salivary glands are free, whereas the 

 hypopharynx is crammed with numerous short trypanosomes of the T. caprce 

 type. In the labial cavity one large colony of large flagellates of a 

 crithidial type was seen. 



In Experiment 2199 the intestine and labial cavity of the infected fly 

 were found to have a heavy infection of trypanosomes. As the intestine was 

 also involved, this is probably a natural infection of the fly with T. pecorum. 

 The animal the flies were fed on was found to be suffering from a T. pecorum 

 infection. 



In the third experiment — 2277 — none of the flies were dissected, but, as 

 the animal the flies were fed on became infected, as in the last experiment, 



