24 



Sir D. Bruce and others. G. brevipalpis as a 



monkey, dog, and goat. Five experiments in all were made ; four negative, 

 one positive. The following Table gives the result : — 



Table II. — Feeding Wild Glossina brevipalpis. 



Date. 



No. 

 of flies 

 fed. 



Monkey. 



Dog. 



G-oat. 



T. hrucei 

 vel 

 rhodesiense. 



T. pecorum. 



g 

 Si' 



<? 



Eh' 



T. hrucei 

 vel 



rliodesiense. 



i 



s 

 J. 



CJ 



a. 

 Eh' 



T. simice. 



a< 

 Eh' 



T. hrucei 

 rel 



rliodesiense. 



s 

 S 



S3 



a. 

 Eh' 



s 



Eh' 



5. 



& 

 e 



Eh' 



1912. 

 Juue 14 ... 



1913. 

 Mar. 18 ... 

 Apr. 29 ... 

 May 7 ... 

 June 25 ... 



42 



146 

 541 

 90 

 276 





+ 









+ 









+ 







Total... 



1095 



























IV. Transmission Experiments. 



Several experiments were carried out with G. brevipalpis, to ascertain if tbis 

 species of tsetse fly can act as a carrier of the various pathogenic trypanosomes 

 found in ISTyasaland. 



These experiments were made, not with laboratory-bred but with wild flies, 

 and this of course takes away much of their value. It was found impossible, 

 on account of the distance from the Lake-shore and the scarcity of flies, to 

 attempt the breeding of G. brevipalpis, in order to obtain laboratory-bred flies. 



The species of trypanosomes experimented with were : (1) T. hrucei vel 

 rliodesiense, the trypanosome causing disease in man in Nyasaland ; 

 (2) T. hrucei, Zululand, 1913 ; (3) T. pecorum ; and (4) T. caprce. 



1. The Development of T. brucei vel rhodesiense in G. brevipalpis. 

 (a) Feeding Wild G. brevipalpis first on Animals infected with the Trypanosome 

 causing Disease in Man in Nyasaland and then on Healthy Animals, to 

 discover if this Species of Trypanosome passes thrmigh a Cycle of Develop- 

 ment in this Species of Tsetse Fly. 

 Two hundred and thirty-two wild G. brevipalpis were used in six experi- 

 ments, but in no case with a positive result. Only fifty-three flies were 

 dissected and eight infected flies found. If all had been dissected probably 

 about 30 to 40 infected flies would have been found. 



