180 Prof. H. E. Armstrong and Mr. H. W. Gosney. 



The effect of glycerol on the synthetic action is similar to that of water on 

 the hydrolytic change, the equilibrium point being so shifted that more acid 

 is removed from the system. Excess of glycerol retards the rate of change 

 in a very noticeable manner. The amounts of acid which entered into 

 combination in a mixture of 4 - 82 grm. of fatty acid and T06 grm. of 

 glycerol, i.e. three molecular proportions of acid to two of glycerol, are shown 

 in the following table. 



Table V. — Synthesis of Fat in Presence of an excess of one Molecular 

 Proportion of Glycerol. 



Time 



Acidity of system 



c.c. normal 



Percentage of acid 



alkali 





combined 



hours 









1 



16-01 



16 -03 



6-2 



2 



15-10 



15 -18 



11 -3 



4 



13-26 



13 -46 



21 -8 



>{ *8 



10 -45 



10 -84 



37 -7 



17 



8-58 



8-51 



49-9 



30 



7-97 



8-17 



52 -7 



50 



7 56 



7 -53 



55-8 



70 



7-57 



7-55 



55 -7 



When three or more molecular proportions of glycerol are present to every 

 three molecular proportions of acid, the retarding effect is so pronounced 

 that no equilibrium point is reached within a reasonably convenient time, 

 the acidity of the system falling slowly after 70 hours. Thus — 



Glycerol, mol. props. 



Acidity after 50 hours 



Acidity after 70 hours 



3 



45 -3 



44-5 



5 



47-3 



42 -3 



10 



44-8 



40 -7 



The effect of glycerol on hydrolysis is similar, as is shown in Table VI, in 

 which is recorded the amount of acid liberated from 5 grm. of olive oil by 

 0-5 grm. enzyme and - 31 c.c. water, in presence of - 53 grm. of glycerol. 



* 



