Studies on Enzyme Action. 



185 



The method is probably one which is affected with a considerable error, so 

 that the results have only qualitative significance. 



Table VII. 



Time 



Glycerol found in 



Percentage 



of acid 

 combined 



Mols. of acid 

 combined 

 per mol. glycerol 



Blank series* 



Series A 



Series B 



hours 



grin. 



grm. 



grm. 







1 



0-51 



0-08 





8-1 



1-6 



2 



52 



Oil 



0-11 



12 -4 



1-8 



4 



0-55 



0-18 



0-19 



23 -9 



2-05 



8 



545 



0-27 



0-28 



33 -8 



2 



17 



0-495 



0-28 



0-28 



37 -9 



2-15 



30 



0-535 



0-295 



0-28 



38 



2 -1 



50 



0-495 



0-28 



0-305 



39 -2 



2 1 



70 



0-515 



0-305 





38 -3 



2-0 



* The amount found should be about "53 grm. 



In the same manner, estimations were made of the amount of glycerol 

 liberated on hydrolysing olive oil by the enzyme in presence of 24 molecular 

 proportions of water. It was found that, at first, the acids liberated were 

 slightly in excess of the glycerol, an indication that a small quantity of a 

 lower glyceride was formed : but as the action continued, the whole molecule 

 was hydrolysed. 



Table VIII. 



Time 



Glycerol found 



Percentage of acid combined 



Mols. of acid 

 liberated 

 per mol. glycerol 



Series A 



Series B 



Series A 



Series B 



hours 



grm. 











1 



0-055 



0-045 



8 -8 



8-5 



2-8 



2 



0-075 



0-065 



15 -9 



13 -8 



3 4 



8 



0-165 



0-165 



36 -2 



34 -9 



3 -4 



17 



0-275 



-25 



56 -2 



53 -0 



3 -3 



30 



0-325 



0-33 



65 -2 



64 -4 



3-1 



50 



-41 



0-395 



77 -5 



76 -3 



3-0 



70 



-43 



0-425 



80 -8 



80 -9 



3-0 



The amount of glycerol liberated, however, is less in proportion to the 

 acid when the hydrolysis is brought about by a small proportion of water, 

 showing that under these conditions mono- and di-glycerides are produced to 

 a greater extent. 



