Dr. W. D. Halliburton. 



Dilution with. 



Coagulation occurred 



2. Globulin from horse serum dissolved 



3. Globulin from hydrocele fluid in 03 



At 14° C. 

 In 46 minutes 



» io „ 



„ 47 „ 



At 40° C. 

 In 20 minutes. 



n 2 „ 

 >, 22 „ 



while a specimen diluted with a saline solution of globulin from serum 

 coagulated in about one-fifth of that time. The explanation of this 

 difference in the action of the serum globulin as derived from these 

 two sources is perfectly clear in the light of the foregoing researches 

 in the ferment powers of cell globulin. 



The serum globulin from hydrocele fluid contains no ferment, 

 because it contains pure serum globulin and no cell globulin. 



The serum globulin obtained from serum contains in addition to 

 the serum globulin that existed in the blood plasma, a certain quantity 

 of cell globulin formed by the disintegration of white corpuscles. 



Both Schmidt and Hammarsten have recognised the fact that the 

 amount of globulin in the serum was greater than in the plasma, and 

 that this extra amount was derived from the white blood corpuscles. 

 The object of this paper is to point out that this extra globulin derived 

 from the white corpuscles is in reality fibrin ferment. I may here 

 mention that examination of the ash of this substance shows that it 

 contains no phosphorus. 



Preparations of serum from which the globulin had been removed 

 by saturation with magnesium sulphate, and the excess of salt by 

 dialysis, were found to have no ferment activity at all. Schmidt 

 found that serum minus its globulin (precipitated by a stream of car- 

 bonic anhydride) has very little ferment activity ; the explanation of 

 its still possessing any is that carbonic acid does not completely pre- 

 cipitate the globulin. When, however, the globulin is completely 

 removed by magnesium sulphate, all ferment activity is completely 

 removed also. 



An extract of " washed blood clot " was found by Buchanan* to 

 hasten the formation of fibrin. Gamgee,f on repeating Buchanan's 

 experiments, concluded that the substance in saline extracts of fibrin 

 which had the powers of fibrin ferment was a globulin ; and this view 

 entirely coincides with the conclusions I have arrived at. In a few 

 experiments in which I have used a 5 per cent, magnesium sulphate 

 extract of fibrin, I obtained in the extract a globulin which has all 



* ' London Medical G-azette,' vol. 18, p. 50. 

 f ' Journal of Physiology,' 1879. 



