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Mr. Gr. F. Dowdeswell. 



dog. In some cases the rabbits died of sapreemia (septic intoxication), 

 to which these animals are extremely liable. 



One rabbit, however, inoculated October 18th, 1886, subcutaneously 

 with virulent medulla, on the twelfth day showed symptoms of 

 infection, with weakness of the hind limbs, the temperature being 

 below the normal and falling. On the fifteenth day it was completely 

 paralysed and died in the afternoon, the post-mortem appearances 

 being highly characteristic, the stomach showing numerous large 

 well-defined haemorrhages, before described, conspicuous from the 

 serous surface as well as on the mucous membrane. 



In another rabbit inoculated in similar manner subcutaneously, the 

 result was precisely the same, excepting that the incubation period 

 was shorter. 



The results of these inoculations in both dogs and rabbits showed 

 conclusively that rabies is produced in both animals, alike by sub- 

 cutaneous and by intracranial inoculation of infective medulla of both 

 dogs and rabbits, confirming M. Pasteur's statements in this respect. 



It was subsequently found that in the rabbits and dogs thus 

 inoculated subcutaneously without producing infection, no protection 

 was afforded against the effect of subsequent intracranial inocula- 

 tion, which in every instance produced fatal infection. 



Still further to dispose of the objection that the symptoms follow- 

 ing intracranial inoculation are not due to specific infection, a rabbit 

 was inoculated sub-durally by trephining with the usual quantity 

 (0*1 c.c.) of mashed spinal cord of a healthy rabbit. The animal 

 remained perfectly unaffected in any way for upwards of a month ; it 

 was then again inoculated intracranially with virulent medulla, by 

 which it was infected, and died after a short incubation period with 

 the usual symptoms and post-mortem appearances. 



I have also made several other inoculations intracranially in rabbits, 

 employing two or three animals at the same time, with medulla of 

 suspected cases of rabies in different animals. In many of these 

 specific infection and death, with typical symptoms and appearances, 

 have followed, but in those cases where the materia 1 used has not been 

 specifically infective, the injections have been perfectly innocuous, the 

 animals being in no wise affected by the operation. 



I have used for intracranial and sub-dural inoculation quantities of 

 medulla, mashed and diluted in bouillon, of from 1 to 10 minims,* 

 with the same results, without, in the large majority of cases, any dis- 

 turbance, previous to or beyond the regular symptoms of infection, 

 following the operation, and without any perceptible difference in the 

 incubation period. Some few animals, especially during the hot 

 weather of August and September, died from accidental causes, 



* The latter quantity, however, is much greater than it is necessary or desirable 

 to use. 



