On Rabies. 



71 



healthy, and were inoculated in the following manner, with medullas 

 of progressively increasing virulence, 0*15 c.c. of the mash prepared 

 as above described being used in each subcutaneous inoculation. 



On the 1st day, morning, cord dried 13 days. 



„ „ evening, „ 11 „ 



,, 2nd ,, ,, 9 ,, 



„ 3rd „ morning „ 6 „ 



„ „ evening, „ 4 „ 



„ 4th „ morning „ 3 „ 



„ „ „ evening, „ 2 „ 



„ 5th „ „ 1 



Of the rabbits thus inoculated, one was found much wasted and 

 partially paralysed behind, with a falling temperature, on the 6th 

 day after the concluding inoculation, it died on the 9th day with well 

 marked and unmistakable post-mortem appearances. 



A second animal died on the 11th day after the last inoculation 

 with symptoms and appearances that clearly showed infection. A 

 third was first affected on the 19th day, and died on the 22nd, clearly 

 of paralytic rabies. 



Two others died some days after the completion of the inoculations 

 with appearances of sapraemia. One remained in good condition and 

 unaffected ; this on the 24th day after the last inoculation, was 

 injected intracranially with 0*1 c.c. of mashed medulla of a rabid 

 rabbit just dead. On the 6th day following, the temperature, pre- 

 viously normal, rose to 40° C, and the following day was the same, 

 with commencing paresis. The symptoms followed the usual course, 

 and the rabbit was found dead on the morning of the 11th day; 

 the duration of the disease — between four and five days — showed the 

 animal to be very robust and healthy, consequently a most favourable 

 subject for protection, but the shortness of the incubation period — 

 the test rightly applied by M. Pasteur to the activity of the virus — 

 proves that it was not in any wise modified by any refractoriness in- 

 duced in the animal by the previous inoculation ; and I think it must 

 be concluded from these experiments that the method followed, essen- 

 tially in accordance with M. Pasteur's last published rapid method, 

 is, as far as rabbits are concerned, inefficient to confer any immunity 

 against subsequent infection, and dangerous as likely to produce it. 



It must, however, be understood that M. Pasteur has not asserted 

 in his communications to the Parisian Academy, that rabbits are 

 capable of being protected. He has confined his statement to dogs. 



Protective Inoculation in the Bog. — The dog should be a far better 

 subject for these experiments than the rabbit, being far more re- 

 sistent to septicaemia and sapraemia, and much less liable to those 



