On Kreatinins. 519 



Determinations of Water of Crystallisation in Urinary Kreatin. 



Weight of air- 

 dried crystals in 

 grams. 



Weight lost at 

 100° C. 



Weight lost by 

 100 parts. 



H 2 in 100 

 parts of 

 C 4 H 9 N 3 2 .H 2 0. 



3-547 

 2-955 



0-4415 

 0-363 



12-45 

 12-28 



12-08 

 12-08 



These results agree with the formula C 4 H 9 N 3 2 .H 2 0. 



Conversion of Urinary Kreatin into Kreatinin Hydrochloride by Liebig's 



Process. 



Weighed portions of the air-dried urinary kreatin were introduced 

 into a counterpoised Liebig's drying tube, connected at one extremity 

 with apparatus for the delivery of dry air or dry hydrogen chloride at 

 will, and at the other extremity with a counterpoised bulb apparatus 

 containing sulphuric acid. In some experiments the kreatin was 

 first dried at 100° C. in dry air, and then subjected to the action of 

 dry HC1 at 100° C. ; in others the crystals were at once acted on by 

 HC1, without previous removal of their water of crystallisation. The 

 final products were found to have similar properties in all the ex- 

 periments. Dry air was, of course, kept passing in all cases at 

 100° C, until no more water was expelled, i.e., until the weight of 

 the sulphuric acid bulbs in front of the kreatin tube became constant. 



The following are some of the quantitative results : — 



Weight of kreatin 

 taken in grams. 



Weight 

 of H 2 

 collected. 



Weight of 

 kreatinin 

 hydrochloride 

 found. 



Theoretical 

 weight of 



H 2 

 expelled. 



Theoretical 

 weight of 

 kreatinin 

 hydrochloride. 



(Cryst.) 1 -066 

 (Anhydrous) 3-1055 

 (Anhydrous) 2 -592 

 (Cryst.) 2-659 

 (Cryst.) 5 -063 



0-276 

 0-412 

 0-364 

 0-594 

 1 -220 



1- 076 

 3-507 



2- 950 

 2-708 

 5-078 



0-259 

 0-426 

 0-356 



0- 642 



1- 223 



1 -0696 

 3-544 

 2-957 

 2-661 

 5 -0799 



The above results sufficiently prove that when urinary kreatin is 

 acted upon by dry HC1 at 100° C, the change is expressed by the 

 equation C 4 H 9 N 3 2 .H 2 + HC1 = C 4 H 7 N 3 0.HC1 + 2H 2 0. 



2 o 2 



