2 Dr. F. W. Mott. Microscopic Changes in the [Feb. 2 1 , 



A comparative examination of the nervous tissues in this disease with 

 that of animals infected with Trypanosoma Gambiense, and with the tissues 

 of human beings dying of chronic Sleeping Sickness, especially those in 

 which there was no evidence of terminal or secondary microbial infection, is 

 of interest in showing that prolonged trypanosome infection causes in all 

 three conditions a marked proliferation and overgrowth of the subpial, 

 septal, and perivascular neuroglia tissue, A chronic interstitial inflammation 

 of the connective tissue structures with lymphocyte infiltration occurs, 

 owing to the presence of an irritative agency in the lymphatic system, which, 

 in the case of Dourine, starting in one seat of primary infection, extends to 

 the inguinal glands, thence presumably by the pelvic lymphatics to the 

 lumbo-sacral plexus and the posterior lumbo-sacral roots to the central 

 nervous system ; consequently the lower part of the spinal cord and especially 

 the posterior column is first and most affected. In the case of Sleeping 

 Sickness there may be any number of seats of infection, but the cervical 

 glands are nearly always markedly involved. 



Material and Notes of Case. — The following portions of the central 

 nervous system of the stallion, hardened in Formol-Muller solution, were 

 examined by various methods to display the neural and neuroglial structures. 

 (1) Brain, lateral side of left lobe. (2) Portion of cervical spinal cord, 

 between third and fourth cervical nerves. (3) Portion opposite the 

 19th nerve (12th dorsal). (4) Portion taken midway between the 22nd 

 and 23rd pair of nerves (15th and 16th dorsal). (5) Portion with 30th pair 

 of nerve roots attached. 



The notes accompanying these tissues were as follows : — 



An account of the Arab stallion (Monarch) will be found in the 

 Appendices, " Keport on Dourine in Different Breeds of Equines, etc.," by Alfred 

 Lingard, M.B., M.S., D.P.H., Imperial Bacteriologist to the Government of 

 India. Page 21. — Infective coitus occurred on May 4 to 6, 1903. Eruption 

 of 156 cutaneous plaques, between June 6, 1903, and August, 1905. Partial 

 paraplegia appeared February 25, 1904. Death (836th day) August 15, 

 1905. 



Post-mortem. — A considerable quantity of gelatinous exudation was found 

 round the lumbar portion of the spinal cord, and a smaller amount around 

 the cervical enlargement, and a certain quantity of cerebro-spinal fluid 

 escaped from within the membranes on removal. 



The cerebro-spinal fluid did not exhibit the Trypanosoma Equiperdum 

 when searched for in numerous stained specimens. 



