242 



Glossina palpalis in its Relation to Trypanosoma gambiense and 

 other Trypanosomes {Preliminary Report). 



By E. A. Minchin, M.A., Professor of Protozoology in the University of 

 London, Lieutenant A. C. H. Gray, RA.M.C, and the late Lieutenant 

 P. M. G-. Tulloch, EA.M.C. (Sleeping Sickness Commission). 



(Communicated by the Tropical Diseases Committee. Received July 10, 1906.) 



[Plates 12—14.] 



Introductory. 



In this paper we propose to give a brief statement of the results obtained 

 by us with reference to the relation of the tsetse-fly (Glossina palpalis) to the 

 trypanosome of Sleeping Sickness (Trypanosoma gambiense), and to other 

 species of trypanosomes which this fly carries. 



Our investigations have consisted of observations and experiments 

 upon (A) Plies fed in the laboratory on animals which had been infected 

 by the inoculation of cerebro-spinal fluid from sleeping sickness patients, and 

 which showed trypanosomes in their blood as the result of such inoculation. 

 (B) Plies caught in various localities which were found on dissection to 

 contain trypanosomes in their digestive tracts. 



Of the trypanosomes under the latter heading (B) we have found two 

 distinct types. One of these types has been named by Professor P. C Novy* 

 Trypanosoma grayi, and one of us (Professor E. A. Minchin) proposes to call 

 the other type Trypanosoma tullocliii. 



Type I. T. tullocliii, n. sp. Minchin. — This type is distinguished by its 

 more rounded nucleus placed near the middle of its body, by a small, usually 

 circular, blepharoplast placed well behindf the nucleus, i.e., at the end 

 furthest from the flagellum (Plate 14, figs. 53 — 60). 



Type II. T. grayi, Novy. — This form is characterised by its large 

 nucleus, which may be oval, spherical, or compressed, and which is in all 

 cases situated not far from the posterior end of the body. In many cases the 

 nucleus sbows distinctly eight chromosomes. The blepharoplast is large, 

 transversely elongated, and situated close to the nucleus, either at its side 



* 1 Journal of Infectious Diseases,' vol. 3, No. 3 (May, 1906), pp. 394—411, Plates 15—17. 

 Professor Novy gives some excellent microphotographs of these trypanosomes, taken from 

 preparations sent him by Lieutenant Gray. 



t In this memoir we use the terms anterior and posterior purely with reference to the 

 direction of locomotion of the trypanosomes described by us, and without prejudice to 

 the disputed morphological questions involved. 



