1906.] Glossina palpalis and Trypanosoma gambiense, etc. 



253 



Appendix I. 



Table I. — List of Animals on which Tsetse-flies known to contain Trypano- 

 somes of the two types mentioned have fed. All these animals remained 

 uninfected by this feeding. 





Number of flies wliicli 



had fed found to 

 contain trypanosomes. 



Class of trypanosome 

 present in fly. 



Presence or absence 

 of trypanosomes from 

 fly's proventriculus. 



"AT/\>-."I-j-\tt "Wr, o'rn 



o 

 & 



J. . grayi. 







o 



» 







o 



£t 







"\T,-» QQ'7 



1 



X 





irresent. 



„ No. 335 



Q 



o 



m " ■ ■ a- 

 J. . grayi m two nies. 



Present in one of the 







T. tullochii in one fly. 



former. 





2 



T. QToyv in one and. 



Present m both. 







T. tullochii in the 









other. 







A. 



T. grayi. 



., . 

 .Absent in all. 



No. 525 



5 



» 



No. 553 



1 





Absent. 



„ No. 554 



4 



?> 



Present in one fly. 



No. 473 



2 



T. grayi in one and 



Present in both. 







T. tullochii in the 









other. 





No. 498 



4 



T. grayi. 



Absent in all. 



„ No. 555 



1 



5) 



Absent. 



No. 556 



3 





Present in two, 



No. 557 



.1 



T. grayi and 



Present. 







T. tullochii together. 





Guinea-pig, I 1 . F 



8 



T. grayi in 7. 



Present in two. 





T. tullochii in 1. 





No. 528 ... 



5 



T. grayi. 



Absent. 



Eat (white), No. 533 ... 



1 



T. tullochii. 



Present. 



Hen No. 505 



4 



T. grayi. 



Present in two. 



Hen No. 506 



6 



T. grayi in 5. 



Present in three. 







T. tullochi in 1. 





Appendix II. — An Experiment on the Cultivation of T. gambiense. 



By Lieutenant A. C. H. Gray, K.A.M.C., and the late Lieutenant F. M. G. 

 Tulloch, RA.M.C. (Sleeping Sickness Commission). 



Our numerous failures in this direction have been attended by one partial 

 success. 



The following method was employed. A tube of agar, prepared according 

 to the formula of McNeal and ISTovy, was melted and cooled to 60° C. Three 

 times its volume of blood, taken directly from the heart of a dog without 

 defibrination, was added to the agar. The water of condensation was 

 inoculated with a drop of blood from a white rat (No. 513) very rich in 

 trypanosomes. On examining the tube six days later a few living trypano- 

 somes were found, which appeared similar to the forms inoculated. On the 



