254 Prof. Mmchin, Lieut. Gray, and late Lieut. Tulloch. [July 10, 



8th and 10th days no trypanosomes were seen in a loopful of fluid with- 

 drawn from the tube. On the 15th day several active trypanosomes were 

 seen in a sample. These trypanosomes were found singly and in groups of 

 three or four. Dividing forms were also seen. These forms were distinctly 

 larger than the trypanosomes originally inoculated, and on measurement were 

 found in some cases to be as long as 54 yx. Besides being longer and broader 

 than the trypanosomes in the blood of the rat, the position of the micro- 

 nucleus was different (Plate 12, fig. 20). In the trypanosomes from the 

 test-tube the micro-nucleus was situated at a considerable distance from the 

 hinder end of the parasite and consequently nearer to the macro-nucleus. 

 These trypanosomes closely resembled certain forms which we have found in 

 the stomach and intestinal tract of tsetse-flies, 24 hours after being allowed 

 to feed on infected monkeys. On the 17th day trypanosomes were still 

 present in about the same numbers, but a few cocci were also found in the 

 tube. Up to the 20th day trypanosomes were still found, but were sluggish 

 in their movements and became fewer in number as the cocci increased. 

 After this date the growth of cocci became profuse and the trypanosomes 

 died off. Up to the present (seven days) no trypanosomes have been 

 found in sub-cultures made from this tube, although the latter are free from 

 bacteria. 



As multiplication had commenced in the original tube, it is reasonable to 

 expect that a successful culture would soon have resulted if it had not 

 become contaminated by cocci. 



The resemblance of the newly formed trypanosomes to forms seen in 

 tsetse-flies after feeding on infected animals is of interest. 



Appendix III. — Some Notes on a Herpetomonas found in the Alimentary Tract 

 of Stomoxys (calcitrans ?) in Uganda. 



By A C. H. Okay, M.B., Lt. E.AM.C. 



In the course of examining the contents of the alimentary tract of some 

 specimens of Stomoxys {calcitrans ?), which had previously been allowed to 

 feed themselves on a monkey infected with the trypanosome of Sleeping 

 Sickness, I found a species of Herpetomonas in the alimentary tract of three 

 flies out of a total number of 280 examined. 



In its movements, size, and general appearance, the flagellate seemed to 

 closely resemble H. muscaz-domesticaz of the common house-fly. 



In two flies this parasite was present in very large numbers. Those two 

 flies were full of blood from the monkey they had fed on 24 hours previously, 

 and in this blood practically unaltered T. gambiense were present in scanty 



