1906.] Glossina palpalis and Trypanosoma gambiense, etc. 



257 



In the third fly non-flagellated forms were found to occur (figs. 8, 9). 

 Masses of blue-staining protoplasm containing chromatin bodies in pairs 

 (fig. 10) were also rarely found in this fly. Every such pair of chromatin 

 bodies consisted of a larger and a smaller separate portion. The larger portion 

 is circular and more faintly staining, the smaller is oval and more deeply 

 staining. These paired masses of chromatin suggest a form analogous to 

 Leishman-bodies. 



On several occasions, in the first two flies, large cells (leucocytes ?) were 

 found containing in their interior the broken-up remains of large numbers of 

 the Hcrpetomonas (fig. 11). 



The figures illustrating these notes are all x 2000 and drawn with the 

 camera lucida from slides fixed in osmic acid and stained with Borrel blue 

 and eosin. 



DESCRIPTION OF PLATES. 



Plate 12. — Trypanosoma gambiense, figs. 1 — 14, forms from the gut of the tsetse-fly 

 {Glossina palpalis) one day after infection, (i.e. about 24 hours after being taken up by 

 the fly. Fig. 15, two days (48 hours) in the fly. Figs. 16 — 19, forms from the blood of 

 a monkey (Cercopithecus sp.) infected with the injection of cerebro-spinal fluid from a 

 Sleeping Sickness patient. Fig. 20, culture form from blood of infected rat, 15th day. 

 All except figs. 14 and 20 preserved wet with osmic vapour stain, Leishman or Geimsa. 

 x 2000. 



Figs. 1 and 2, male form with compressed nucleus. Fig. 3, male form with rounded 

 nucleus. Figs. 4 and 5, similar forms with chromatin being given off from the nucleus. 

 Fig. 6, male form dividing, chromatin being given off from both the daughter nuclei. 

 Figs. 7 — 14, female forms, figs. 7 and 12 dividing. Fig. 15. indifferent form. Fig. 16, 

 male form. Fig. 17, female form, very scarce (only one other was found). Figs. 18 and 

 19, indifferent forms which in the fly become female ; note the short, free flagellum. 

 Fig. 20, female form from culture tube, 15th day. 



Plate 13. — Trypanosoma grayi, .figs. 21 — 30, from the gut of the tsetse-fly (Glossina 

 palpalis). The flies from which these trypanosomes were obtained had been fed regularly 

 on the blood of neutral monkeys and were dissected after about 10 days of such feeding 

 In all cases the whole gut of the fly swarmed with thousands of similar trypanosomes. 

 Figs. 31 and 32, small forms ; flies dissected 24 hours after their first feed of blood 

 generally contained forms such as these in very great abundance, many dividing forms 

 were also present, larger forms, as above, were rare. Figs. 33 — 35, trypanosomes from 

 fresh-caught tsetse flies, which had not fed on blood for a long while. In such flies, 

 parasites were never very numerous and the types present were all of a large size. 

 Figs. 36 — 40, various other types from flies which had fed on blood. Fixed in alcohol, 

 stained with Leishman or Giemsa. x 2000. 



Figs. 21 and 22, male forms with compressed nucleus and long free flagellum. Figs. 23, 

 24 and 25, female forms. Posterior extremity thickened, short free flagellum. Figs. 26, 

 27 and 28, stages of division showing the unequal size of the two resulting individuals. 

 Fig. 29, division of a small form into two more or less equal-sized individuals. Fig. 30, 

 aberrant dividing form, in this case the nucleus has already divided, whereas the 

 blepharoplast has not yet done so. Figs. 31 and 32, young forms resulting from the 

 unequal division of a large female form. Fig. 33, very long male form. Fig. 34, 

 VOL. LXXVIII. — B. X 



