306 Drs. D. A. Welsh and H. G. Chapman. On the [Apr. 30, 



the result is to show that in the final tube A there was no great residue of 

 precipitin, whereas in tube B there was still sufficient homologous proteid to 

 give the maximum precipitate from - 01 gramme of this antiserum. This 

 amount of homologous proteid could not exceed one-tenth of that orginally 

 present, that is, it could not exceed 0-00006 gramme, and had already been 

 acted on five times. These results are summarised in Table VIII : — 



Table VIII. — Experiment V. 



No. of 

 interaction. 



Amount of egg white remaining 

 at each stage. 



Total 

 amount of 



fluid at 

 ekch stage. 



Amount in 

 grammes of 

 antiserum 

 added at 

 each stage. 



Duration 

 of each 

 interaction. 



Precipita 

 at end of 

 each stage. 





gramme. 



c.c. 





hrs. 



mm. 



1 



O-0006 



5 



0-01 



48 



0-5 + 



2 



0004S (once acted on) 



4 



o-oi 



48 



0-5 + 



3 



-00036 (twice acted on) 



3 



o-oi 



48 



1 



4 



'0003 (three times acted on) . . . 



2-5 



o-oi 



48 



2-5 



5 



-00018 (four times acted on) ... 



1-5 



-oi 



72 



2 



6 



'00006 (five times acted on) . . . 



5 



o-oi 



48 



2 



In Experiment VI a much smaller amount (equivalent to 0-000000012 

 gramme dried) of egg white was originally taken and was allowed to react 

 with 0*01 gramme dried antiserum (prepared in a rabbit with dried egg 

 white). At the end of 48 hours the precipitum was noted, the superfluid was 

 filtered, and a fresh supply of O'Ol gramme antiserum added. This process 

 was repeated again and again, as summarised in Table IX. The precipitates 

 were distinctly flocculent : — 



Table IX.— Experiment VI. 



No. of 

 inter- 

 action. 



Amount in grammes 

 of egg-white 

 remaining at each 

 stage. 



Amount 

 of fluid 

 at each 

 stage. 



Grammes of 

 antiserum 

 added 

 at each 

 stage. 



Duration 

 of each 

 inter- 

 action. 



Precipita at end of 

 each interaction. 







c.c. 





hrs. 



mm. 



1 



-000000012 



5 



o-oi 



72 



slight = 0-3 



2 



-0000000096 

 (once acted on) 



4 



o-oi 



48 



„ =0-3 



3 



-0000000072 

 (twice acted on) 



3 



o-oi 



48 



„ =0-3 



4 



-0000000048 

 (three times acted on) 



2 



o-oi 



72 



„ =0-3 



5 



-000000003 

 (four times acted on) 



1 25 



o-oi 



48 



more abundant = '5 



6 



-0000000018 



0-75 



o-oi 



48 



= 1 





(five times acted on) 











