416 Messrs. G. A. Buckmaster and J. A. Gardner. [July 11, 



employed by Pohl for determining the quantity of chloroform in blood 

 during narcosis is similar, except that magnesia was substituted for lime. 



The chief results of Pohl's determinations are that pure water in contact 

 with chloroform takes up 0*794 per cent, at 15° C, which closely approxi- 

 mates to the figure obtained by Chancel and Parmentier,* 0*987 at 0° C, but is 

 higher than the figures obtained by Moore and Koaf,f who find that at 17° C, 

 with 0*04957 per cent, of chloroform vapour in a space where the vapour 

 exerts a pressure of 74 - 61 mm. of mercury, the percentage by weight of 

 chloroform taken up by distilled water is 0"586 ; that the amount recoverable 

 from the blood of anaesthetised animals is much less than this, namely, 

 0*029, 0*018, 0'05 per cent, of chloroform, and that solutions of hemoglobin 

 in water do not take up more chloroform than the same volume of water, 

 though an alcohol and ether extract of blood takes up as much as 

 1*105 per cent. 



During narcosis in dogs Pohl obtained the following figures for arterial 

 blood : — 



Grammes of CHC1, 

 per 100 grammes 

 of blood. 



5 mins. after the commencement of administration ... 0*009 

 8 „ „ „ „ ... 0*018 



60 „ „ „ „ ... 0*026 



Death from chloroform 0*035 



Blood from right ventricle 0*058 



left ventricle 0*027 



In another experiment in which both the cardiac and respiratory move- 

 ments had ceased in a dog weighing 32 kilogrammes, in the blood of the 

 right ventricle, 0*042 gramme per 100, and in the blood of the left ventricle, 

 0*058 gramme per 100 of chloroform were found. 



The chief conclusions which Pohl drew from his experiments were 

 that : — 



(1) The anaesthetic dose of chloroform in the arterial blood of dogs was 

 0'029 or 0*018 or 0*05 gramme of chloroform per 100 grammes of blood; 



(2) The average amount which was present in the blood of animals killed 

 with chloroform was 0*035 gramme per cent. 



(3) The lethal and anaesthetic dose of chloroform closely approximate one 

 to the other. 



* ' Comptes Rendus,' vol. 100, p. 773, 1885. 

 t ' Roy. Soc Proc.,' vol. 73, May 5, 1904. 



