1906.] 



Chloroform in the Blood of Animals. 



419 



Weight in kilogrammes. 



Anaesthesia. 

 Milligrammes per 100 grammes. 



23 

 8 



18 

 9 



54 

 50 

 58 

 57 



After anaesthesia has been induced and the supply of chloroform is stopped, 

 this drug rapidly leaves the blood. Five minutes after anaesthesia half the 

 amount disappeared ; three hours later 7 milligrammes were found in the blood, 

 which became free from chloroform seven hours after narcosis. 



Using centrifugalised oxalated blood removed from the inferior vena cava, 

 Nicloux recovered 64 - 4 per cent, of the chloroform from the red corpuscles 

 and 13 - 3 per cent, from the plasma. However, the volume of the corpuscles 

 was high in both the experiments he has described, being in one case 

 14 c.c. of plasma to 26 c.c. of deposit, and in the other 15 c.c. of plasma 

 to 25 c.c. of corpuscles. His figures of the distribution of chloroform in 

 blood are much below those of Pohl, who has stated that 87 to 90 per cent, 

 of the total chloroform is held by the red corpuscles, that is the chloroform- 

 holding power of the corpuscles is 7 to 8 times that of the plasma. The 

 latter figures, as will be shown subsequently, are more in accord with our own 

 observations. 



The results obtained by J. Tissot show that: — 



(1) 34 to 40 milligrammes of chloroform in 100 grammes of blood is 

 sufficient to produce anaesthesia (Tissot), or 32 to 43 milligrammes (Mansion 

 and Tissot). 



(2) When mortal syncope is produced in dogs with very slow inhalation of 

 small percentages of CHCI3, the following amounts of chloroform are found 

 in arterial blood : — 



Just prior to mortal syncope the amount of chloroform in blood falls 

 thus : — 



Milligrammes per 100 grammes. 



Milligrammes per 100 grammes. 



96 

 881 

 84-2 

 59-8 



60 

 67-2 

 105-2 

 77-1 



Milligrammes per 100 grammes. 



65 



62-5 



54-5 



Milligrammes per 100 grammes. 

 62-2 

 56-05 



