1906.] 



Chloroform in the Blood of Animals. 



427 



Experiment 3. — We quote this experiment as an illustration of the greatest 

 difference in the chlorine content of the blood ever noticed. In this particular 

 experiment a large quantity of blood, about 23 c.c, was withdrawn during the 

 interval between the times of taking the samples analysed. The cat weighed 

 3 - 8 kilogrammes. The results are given in Table III. 



Table III— April 13, 1906. She Cat. Weight, 3-8 kilogrammes. 



Time. 



Anaes- 

 thetic. 



Weight 



of 

 blood 

 in gr. 



Weight 



of 

 AgCl. 



CHC1 3 

 per cent. 



Mean 

 value of 

 chlorine 

 per cent. 



Deviation 

 from mean. 



Average 

 deviation 

 from mean. 



Eemarks. 



P.M. 



12.15 

 12.48 



Ether on 

 jj 



4 -2378 



-0572 



-33273 







-0-00525 









12.50 

 1.4 





3 -9088 



-0543 



-34323 





> 0-33798 



+ 0-00525 



-> 



-0-00525 



18 c.c. blood taken 

 from animal, making 

 altogether about 

 23 c.c. 



Animal nearly as- 

 phyxiated. It could 

 not be brought round 



Experiments to Determine the Amount of Chloroform in the Arterial Blood of 

 an Animal at Different Stages of Chloroform Narcosis. 

 Experiment 4. — This was one of the earliest experiments performed. The 

 blood was taken from the left carotid. The chloroform was administered by 

 means of a Woulff's bottle, and after the animal was fairly deeply 

 anaesthetised it was kept as far as possible under constant conditions. The 

 results are given in Table IV. 



Table IV. — Cat. Left carotid tapped. 



Time. 



Anaesthetic. 



Weight 

 of 

 blood 

 in gr. 



Weight 



of 

 AgCl. 



Per- ' 



centage 

 of 



chlorine. 



Difference from control — 



Eemarks. 



As 

 chlorine. 



As 

 CHC1 3 . 



As 

 vapour 

 of CHC1 3 

 at 0° C. 



and 

 760 mm. 



P.M. 



12.30 

 1.0 



1.0* 

 1.9 

 1.14 

 1.19 



Ether started 

 Ether 



5 -0843 



3 -8387 



4 -5512 



5 -6276 



-0677 



-0534 

 -0645 

 -0796 



-3282 



"3429 

 -3493 

 -3487 



-0147 

 0-0213 

 -0206 



-0165 



0-024 



0-023 



2 -56 

 4-5 \ 

 4-3 J 



Sample taken -when 

 reflexes just gone 



Reflexes going 



Animal maintained 

 in the same state 

 of anaesthesia 

 throughout 



Chloroform on 



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