428 Messrs. G. A. Buckmaster and J. A. Gardner. [July 11, 



Experiment 5. — In this experiment a male cat, weighing 4 kilogrammes, 

 was used, and the blood was taken from the right carotid. The chloroform 

 was administered from bags filled by means of the Dubois apparatus, with 

 approximately a 2-per-cent. mixture of chloroform vapour and air. The 

 animal was gradually anaesthetised until respiration ceased, when the chloro- 

 form was cut off and the cat allowed partially to recover. It was then kept 

 under light ether anaesthesia until 5.5 p.m., 29 minutes after the chloroform 

 was stopped, when a final sample of blood was drawn. The samples of blood 

 while the animal was under chloroform were taken every 10 minutes, and 

 the respirations were recorded. The darkness of the colour of the blood 































































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2-30p.m. 



5 p.m . 

 Ether 



4 p.m. 



2%chloroform 



Reflexes 

 marked 



A. 



Constructed from Experiment 5. Samples of arterial blood ( + ). 



increased with the percentage of anaesthetic in the blood, and appeared to 

 afford a good criterion of the progress of the anaesthesia. The results of this 

 experiment are given in Table V and Curve A. After the chloroform was 

 cut off the anaesthetic was rapidly eliminated from the blood, and 14 minutes 

 later the reflexes were well marked. At the time of taking the final sample 

 the greater portion of the chloroform had disappeared. 



Experiment 6. — For this experiment a female cat, weighing 4'3 kilogrammes, 

 was taken. The right carotid was tapped, and the chloroform administered 

 by means of a Woulffs bottle. The control sample was taken when the 

 reflexes were just visible. The chloroform was then administered, and samples 



