1906.] 



Chloroform in the Blood of Animals. 



431 



taken when the conjunctival and tail reflexes had entirely disappeared, five 

 minutes later, and when respiration just ceased. The chloroform was stopped 

 at this point, and the animal allowed to recover. In this case artificial 



Reflexes RespiraF!' Reflexes AniraJ 

 gone ceased well ceased 

 entirely nu/ted bo 



respire 



B. 



Constructed from Experiment 6. 



respiration had to be resorted to before the cat began to breathe naturally. 

 A sample of blood was collected when the reflexes had just entirely 

 reappeared. After this stage chloroform was again administered until the 

 animal was on the point of asphyxiation, when the final sample of blood was 

 taken. It will be noticed that in this experiment also the colour of the blood 

 kept pace closely with the amount of anaesthetic in the blood. After the 

 final sample had been taken the animal was revived again by the use of the 

 bellows, and the blood appeared bright in colour. The amounts of chloroform 

 per cent, of blood were practically identical at the two points where 

 respiration ceased. The results of the experiment are recorded in Table VI 

 and Curve B. 



Experiment 7. — In this case a male cat, weighing 3 - 5 kilogrammes, was 

 taken and the right carotid tapped. The chloroform was administered from 

 bags filled with mixtures of chloroform vapour and air by means of the 

 Dubois apparatus. At first 2-per-cent. chloroform mixture was used, and 

 samples of blood were drawn after the reflexes had entirely vanished and 

 when the animal was very deeply under. Three-per-cent. chloroform mixture 

 was then substituted for 2-per-cent., and a sample taken when the animal 



