1906.] 



Chloroform in the Blood of Animals. 



433 



Ether 

 Time in seconds 



2% chloroform 

 n A 

 Reflexes 

 quibe gone 



C. 



z- 55 p.m . 

 3%CHC1 3 4-5% 



Reflexes 

 quite marked 



Constructed from Experiment 7. 



showed signs of asphyxiation. During the administration of the 3-per-cent. 

 mixture the respiration was shallow and irregular, and of the Cheyne-Stokes 

 type. The animal was then allowed partially to revive, and a sample of blood 

 was collected when the reflexes were well marked. Chloroform was now 

 administered again, the mixture of chloroform vapour and air used being of 

 approximately 4 - 5 per cent, strength. The reflexes took rather a long time 

 to entirely disappear, and the time of disappearance was rendered more 

 difficult to ascertain by the complication of clonic twitching of the hind 

 limbs. These movements vanished before respiration ceased, when a sample 

 of blood was collected. 



The results of the experiment are given in Table VII and Curve C. It 

 will be noticed that the amounts of chloroform in the blood at the two 

 stages of the experiment at which respiration ceased are almost identical. 



Experiment 8. — In this experiment a female cat, weighing 2 - 7 kilogrammes, 

 was taken and the chloroform was administered from bags fi]led with air, 

 containing approximately 2 per cent, of chloroform vapour, by means of the 

 Dubois apparatus. The object of this experiment was to obtain two curves 

 showing in the one case the proportions of chloroform in the blood at various 

 stages up to the asphyxiation point immediately after ansesthetisation by 

 ether, and in the other case the amounts at the various stages in a second 

 chloroform experiment, after the animal had recovered from the first. 



After the control sample had been taken under ether, the cat was allowed 

 so far to recover that the reflexes were well marked, and then the chloroform 

 was administered. Samples of blood were collected when the reflexes had 



