438 Messrs. G. A. Buckmaster and J. A. Gardner. [July 11, 



was allowed to recover until the ether anaesthesia was very light. The chloro- 

 form bottle was now connected to the Chauveau valve, and the anaesthetic 

 administered fairly gently for 14 minutes, after which it was pushed by 

 lowering the inlet air tube to the surface of the chloroform, and a sample of 

 blood was collected when respiration ceased ; the blood at this stage was very 

 dark in colour. During the period in which the anaesthesia was pushed 

 curious alternating rhythmical movements of the hind limbs were noticed. 

 The cat was allowed to recover naturally, and a sample of blood collected 

 when the reflexes were slightly marked. The colour of the sample was bright 

 red. Thirty-two cubic centimetres of blood were now withdrawn from the 

 animal, making in all since the control sample about 47 to 50 c.c. This 

 quantity was probably a little over 1/3 of the total blood in the animal. 



The chloroform was now administered again, and, after the first few 

 minutes, was pushed until respiration stopped, when a sample, very dark in 

 colour, was collected. During the later stages of this anaesthesia, the above- 

 mentioned rhythmical movements of the limbs were again noticed, and they 

 ceased a little before respiration stopped. 



The results of this experiment are recorded in Table IX and Curve E. It 

 will be noticed that the amount of chloroform in the blood at the asphyxia- 



u. 



bo IO 



Q_ 



W T3 



6 



5 



o Z 

 % 



I 



o 

 o 













































































































































i 















































\ 

 \ 



\ 



\ — i 























































n 



35 







1 v — 



v — 











=¥= ! — 





on 



E. 



pushed 



l 5 CHC1, 



pushed A A on 



Respira c - n Slight Reflexes 

 ceased reflex fully marked 

 movements 



Constructed from Experiment 9. Samples of arterial blood ( + ). 



