446 Messrs. G. A. Buckmaster and J. A. Gardner. [July 11, 



much more easily in the ease of cat's blood than in the case of dog's. The 

 samples of corpusles and plasma were analysed in the same way as before. 



"With the centrifugal machines at our command we found it very difficult 

 to get exactly equal ratios of separation of red matter and plasma in the 

 control samples taken under ether and the samples taken under chloroform. 

 As the percentage of natural chlorine in the red corpuscles is very different 

 from that in the plasma it is obviously impossible to calculate with any 

 degree of accuracy from the experimental results the exact distribution of 

 chloroform between the corpuscles and plasma, unless the ratios of separation 

 in the control and chloroform samples are sensibly equal. With very great 

 care we succeeded in getting a sufficiently equal separation in a few 

 experiments to permit of this calculation being performed with accuracy. In 

 the other experiments, though the calculation could not be made, the results 

 obtained indicated clearly that the chloroform associated itself with the 

 corpuscles rather than the plasma. 



Experiment 12. — In this experiment a cat was anaesthetised in the way 

 described, using a Woulff's bottle. Samples of blood were collected under 

 ether, and also when the animal was very deeply under the influence of 

 chloroform. Clotting was prevented by oxalate. Unfortunately the Carius 

 tubes containing the corpuscles exploded, but the percentages of chlorine 

 found in the control sample and the chloroform sample were respectively 

 - 3819 and 03895. This was one of our earlier experiments, and the silver 

 chloride was separated by filtration through paper and weighed in the usual 

 manner instead of by the method described at the beginning of this paper; 

 the results are, therefore, approximate, and we give them for what they are 

 worth. They show, however, that little, if any, of the chloroform administered 

 was in the plasma. 



Experiment 13. — For the purpose of this experiment a cat weighing 

 2 - 8 kilogrammes was taken, the chloroform being administered by means of a 

 "Woulff's bottle, and the blood collected when the animal was deeply under. 

 The samples of blood were centrifugalised in ice without the addition of 

 oxalate. The ratios of separation in the control and chloroform samples were 

 not, however, equal. The chlorine was estimated by the method used in the 

 previous experiment, so that we do not regard the analysis as possessing the 

 highest degree of accuracy. 



The following Table XIII (p. 447) gives the result obtained. 



Whereas the percentage of chlorine in the corpuscles shows an increase of 

 - 056, that in the plasma is only - 005. 



Experiment 14. — In this experiment a dog was anassthetised with ether and 

 a control sample of blood taken. Chloroform was then administered by 



