1906.] Chloroform in the Blood of Animals. 447 



Table XIII. 





Weight of 

 corpuscles. 



Weight of 

 plasma. 



Weight of 

 silver 

 chloride 

 from 

 corpuscles. 



Weight of 

 silver 

 chloride 



from 

 plasma. 



Percentage of 

 chlorine. 



In 



corpuscles. 



In 



plasma. 



Control expt., under ether 

 Expt., under chloroform 



2 7095 

 1 -7798 



2 -8975 



3 -4678 



-0342 

 -0268 



0-0496 

 '0587 



0-315 

 0-371 



-423 

 -428 



means of a Woulff 's bottle, and three samples of blood collected at various 

 stages of anaesthesia up to the asphyxiation point. An attempt was made to 

 centrifugalise the blood simply cooled in ice without the addition of oxalate, 

 but all the samples clotted. They were, therefore, allowed to stand until 

 contraction took place, when they were again centrifugalised, and the 

 corpuscles and serum analysed. The analysis was made by the same 

 method as in Experiment 13. The ratios of separation of corpuscles and 

 serum in the various samples were not equal, so that ratios of chloroform 

 distribution could not be calculated. The results, however, indicate that 

 practically no chloroform went into the plasma, except when the anaesthesia 

 was pushed to an extreme point. The results are given in the following 

 Table. 



' Table XIV. 





Weight of 

 corpuscles. 



Weight of 



silver in 

 corpuscles. 



Per- 

 centage of 

 chlorine in 

 corpuscles. 



Weight of 

 serum. 



Weight of 

 silver 

 chloride 

 in serum. 



Per- 



centage of 

 chlorine 

 in serum. 



Control samples under 

 ether 



Under CHC1 3 when 



reflexes gone 

 Under CHC1 3 when 



breathing was very 



feeble 



3 -5942 

 3 -3118 

 2 -8333 



-0376 

 -0378 

 -0367 



-254 

 0-282 

 0-319 



2 -042 

 2 -258 

 2 -2084 



-0321 

 -0355 

 -0373 



-3875 

 -3876 

 -4173 



Experiment 15. — In this experiment a cat weighing 31 kilogrammes was 

 taken and anaesthetised with ether; 10 c.c. of blood were then withdrawn 

 when the reflexes were scarcely marked, and mixed with \ c.c. of saturated 

 sodium oxalate. The sample was then divided into two equal portions, each 

 of which was weighed and centrifugalised. One sample was analysed and 



