448 Messrs. G. A. Buckmaster and J. A. Gardner. [July 11, 



the other left for experiments which will be mentioned later ; the animal was 

 then allowed to recover partially, and chloroform was administered slowly by 

 means of a Woulff 's bottle. Samples were collected when the reflexes had 

 just gone, and when respiration was on the point of ceasing. The samples 

 were treated in the same way as the control sample. The analyses were 

 made with the utmost care. The results are recorded in Table XV. 



It will be noticed that the percentage of chlorine in the plasma remained 

 constant, at any rate to within the probable errors of experiment. Calculation 

 shows that 71 and 73 per cent, of the chloroform was taken up by the red 

 corpuscles. Probably, however, a much higher proportion than this, perhaps 

 the whole, was taken by the corpuscles, as the percentage of chlorine in the 

 plasma remained the same. The discrepancy can, we think, be quite 

 accounted for by the fact that the ratios of separation of corpuscles and 

 plasma in the various samples were not quite equal. 



Experiment 16. — In this experiment the samples of blood used were taken 

 from the dog used in Experiment 10, chloroform being administered by the 

 Dubois apparatus. The results are given in Table XVI. 



Here again the bulk of the chloroform was absorbed by the red matter, and 

 little went into the plasma. Calculation shows that at the point of the 

 vanishing of the reflexes 64 per cent, attached itself to the corpuscular matter, 



Table XV.— Cat. 



Time. 



Anesthetic. 



Weight 

 of blood 

 + 0-25 

 c.c. 

 oxalate 

 of soda. 



Weight 



of 

 AgCl. 



Total 

 percent- 

 age of 

 chlorine. 



Difference from control — 



As 

 chlorine. 



As 

 CHCLj. 



As 

 vapour 

 ofCHCl 3 

 at 0° C. 



and 

 760 mm. 



Weight 

 of cor- 

 puscles. 



Per- 



Weight cent , a S e 



ojf 



, m chlorine 

 AgCl. 

 ° m cor- 



puscles. 



A.M. 

 10.45 

 11.20 



11.20 

 11.3-1 



11.40 

 11.46 

 11.53 

 11.54 



Ether began. 

 Ether 



•1975 



-0685 -3249 



CHC1, on. 



5-1823 0-0719 0-3420 



CHC1 3 pushed. 



5 -2267 -0760 



-35844 



0-01714 0-01924 3-6 



0-0336 0-0377 | 7-07 



1 -9781 -0184 -2293 



1 -9439 -0209 -2650 



1-8804 | 0-0237 0-3107 



Percentage of chloroform taken by corpuscles 



;> jj 3> )> 



