1906.] 



Chloroform in the Blood of Animals. 



449 



but in this case the calculation is vitiated even more than in the previous 

 experiment by inequality in the ratios of separation, which were T034 in the 

 control and T048 in the chloroformed sample later. 



Experiment 17. — For the purpose of this experiment a cat weighing 

 3 - 5 kilogrammes was taken. A control sample of 10 c.c. was taken and 

 mixed with £ c.c. sodium-oxalate solution and centrifugalised. The cat after 

 partial recovery was then slowly chloroformed, using bags filled with air 

 containing approximately 2 per cent, of chloroform vapour, by means of the 

 Dubois apparatus. A 10 c.c. sample of blood was collected when the animal 

 was very deeply under the influence of the anaesthetic. The colour of the 

 sample was very dark. It was treated exactly as in the case of the control. 

 The samples, which were larger than in other experiments, were analysed 

 with the utmost precaution. In this case we were fortunate enough to get a 

 very good separation of corpuscles and plasma, and the ratios in the case of 

 control and chloroform samples were very nearly equal. 



The results are given in Table XVII. In this experiment the whole of the 

 chloroform associated itself with the red corpuscles. 



A number of experiments were performed, the results of which are not 

 recorded, as, owing to accidents in the analysis, they did not form a complete 

 series. The results obtained, however, were in accordance with those quoted. 



Weight, 3T kilogrammes. 





Per- 



centage 

 of cor- 

 puscular 

 chlorine 



per 

 cent, of 

 blood. 



Difference of corpuscular 

 chlorine per cent, of blood. 







Per- 

 centage 



of 

 chlorine 



in 

 plasma. 







As 

 chlorine. 



As 

 CHC1 3 . 



As 

 vapour 

 ofCHCl 3 

 at 0° G. 



and 

 760 mm. 



Weight 



of 

 plasma. 



Weight 



of 

 AgCl. 



Remarks, 





-0873 









3 -2194 



-0501 



"3836 



Sample taken when reflexes just present. 

 Respiration 32 per min. 





-0994 



-0121 



-0136 



2-56 



3 -2384 



-0510 



-3882 



Sample taken when reflexes had just 

 completely gone. Respiration 44 per 

 min. 





-1118 



-0245 



-0275 



5-17 



3 -3463 



-0523 



-3853 



Respiration 28 per min. 

 Respiration 48, and very shallow 

 Kespiration 20, and very shallow. Colour 

 of blood very dark 



7l - l, at period of vanishing of reflexes. 

 73'1, at period of asphyxiation. 



