452 Messrs. G. A. Buckmaster and J. A. Gardner. [July 11, 



in vacuo over sulphuric acid, and finally heating for some hours in the steam 

 oven. 



Experiment 18. — In this experiment the samples were taken from the dog 

 used in Experiment 10. The chlorine values obtained by direct analysis 

 without drying are those quoted in Experiment 16. 



In the following Table we give the percentages of chlorine found in the 

 undried and dried samples of corpuscles and plasma : — 





Percentage of chlorine. 



Corpuscles. 



Plasma. 



Undried. 



Dried. 



Difference. 



Undried. 



Dried. 



Difference. 



Control samples under 

 ether 



Sample under CHCL, 

 when reflexes had gone 



Sample under CHC1 3 

 when blood was very 

 dark in colour 



-1993 

 -2221 

 -2660 



-1811 

 -1885 

 -2027 



-0182 

 -0336 

 -0633 



-3477 

 -3566 

 -3636 



-3284 

 -3223 

 -3188 



-0193 

 -0343 

 -0448 



We were surprised to find that the control sample both of corpuscles and 

 plasma lost chlorine on drying ; this loss was more marked in the case of the 

 plasma. An inspection of the Table, however, will show that the chloroform 

 samples lost considerably more chlorine, and that the figures obtained after 

 drying are almost identical in each set. All the chloroform therefore 

 appeared to be eliminated on drying. 



Experiment 19. — The results quoted in this experiment were obtained with 

 the blood of the animal mentioned in Experiment 15, which was treated in 

 the same manner as described in Experiment 18, but the heating was not 

 so prolonged, and the temperature was not above 90°. 





Percentage of chlorine. 





Corpuscles. 



Plasma. 





Undried. 



Dried. 



Difference. 



Undried. 



Dried. 



Difference. 



Control under ether . . . 



-2293 



-2297 





-3836 



-3511 



-0325 



CHC1 3 when reflexes 



-2650 



-2486 



-0164 



-3882 



-3518 



-0364 



gone 



CHCI3 very deeply 

 under 



-3107 



-2854 



-0253 



-3852 



lost 





