478 



Drs. Herring and Simpson. Relation of the [May 31, 



amount of extravasation of the carmine gelatine, principally in the connective 

 tissue accompanying the blood-vessels and bile ducts, but also in some of 

 the lobules. Injection mass is also seen in the walls of the hepatic veins. 



Microscopical examination of sections lightly stained with hsematoxylin 

 shows that the carmine gelatine is in the connective tissue of the liver, and 

 that large injected trunks accompany the branches of the hepatic artery, 

 portal vein, and bile ducts. In the large portal spaces there is frequently 

 extravasation throughout the connective tissue ; the large lymphatic trunks 

 can be distinguished by their being filled with carmine gelatine, but the 

 surrounding connective tissue is lightly stained owing to the presence in 

 it of diluted injection mass.* The carmine gelatine is frequently seen 

 extending into some of the lobules even to the central vein, and presents an 

 appearance very like that described by MacGillavry. In other places no 

 trace can be seen of injection passing into the lobules, although extravasation 

 has occurred throughout the connective tissue bordering them ; a sharp line 

 of demarcation exists between injected connective tissue and non-injected 

 periphery of lobule. 



In the smaller portal spaces injected lymphatics are sharply denned and 

 extravasation is uncommon. Lymphatics are often seen running parallel 

 with and close to branches of the hepatic artery ; they also surround the 

 bile ducts and portal vein. Even in the smallest portal spaces lymphatics 

 are found (fig. 14). The walls of the hepatic veins contain a large plexus 

 of lymph vessels lying principally in the adventitia close to the liver 

 substance, but there may be several layers with anastomosing branches 

 (fig. 16). The mode of connection between the portal lymphatics and 

 those of the hepatic veins is easily seen. Branches of the hepatic artery 

 supply the walls of the hepatic veins, and lymphatics accompany these 

 branches. Further, large portal spaces containing hepatic artery, portal vein, 

 and bile ducts are found in close connection with, indeed joined on to, the 

 walls of the hepatic veins (fig. 13). There is, in fact, an intimate relation- 

 ship between Glisson's capsule and the adventitia of the hepatic veins, and 

 the large lymphatics of the two systems are directly continuous in these 

 situations. The place of entry of the branches of the portal vein into the 

 liver is, moreover, very close to the large hepatic veins, and the lymphatics 

 of the latter join the large lymphatics which accompany the branches of the 

 portal vein and which leave the liver by the portal fissure. Disse, alone 

 of all who have previously worked at the subject, described this connection 

 between the two systems. Budge did not notice it, and was led to believe 



* Is it possible that on dilution with alkaline lymph some of the carmine becomes 

 diffusible ? 



