88 



Prof. B. Moore and Dr. H. E. Koaf. [Oct. 19, 



case of all the tissues experimented with the solubility is much higher than 

 in the case of water or saline. 



As in the case of serum and haemoglobin, a gradual precipitation of the 

 tissue proteid occurs with increasing concentration of chloroform, shown by 

 change in colour and by the emulsion of tissue becoming flocculent and 

 separating out. This change occurs more readily, and at lower concentration 

 of chloroform, at body temperature than at room temperature. This is shown 

 by the fact that a mixture of the tissue and a certain strength of chloroform 

 which remains perfectly stable and uncoagulated when kept unheated, rapidly 

 changes colour and separates out when heated in the densimeter. 



The amount of proteid in each tissue emulsion was determined by 

 coagulation by excess of alcohol, drying and weighing ; and the amount 

 of ethereal extractive, by extracting in the manner described in the next 

 section for preparation of ethereal extracts. The amount of ethereal extract 

 so obtained was used to determine the amount of ethereal extract of the 

 fresh tissue required to be added to saline in order to form an emulsion of 

 equal concentration in lipoid for carrying out the companion vapour-pressure 

 experiments of the succeeding section. 



Experiment 1. — Brain Tissue. 



The brains of two sheep were prepared as above described. The amount of 

 proteid in the emulsion as introduced into the densimeter was 7"36 per cent., 

 and the amount of ethereal extractive was 4 - 07 per cent. The results of the 

 experiments are given in Table I, and graphically in Curve 1, fig. 1, in which 

 they are compared with ethereal extractive alone, of equal concentration, in 

 emulsion in normal saline. See Experiment 5 of next section. 



Table I.— Temp. 40° C. 



Percentage by 

 weight of 

 chloroform 

 originally 

 introduced. 



Pressure of 

 chloroform in 

 vapour space, 

 in mm. of 

 mercury. 



Percentage by 

 weight of 

 chloroform 

 pumped off into 

 vapour space. 



Percentage by 

 weight of 

 chloroform 

 remaining in 

 solution. 



Coefficient of 

 distribution 

 between 



vapour space 

 and solvent. 



0-05 



6-21 



-0038 



-0462 



1 : 12 -2 



o-i 



13 -14 



-0081 



-0919 



1 : 11 -4 



0-2 



26 25 



-0162 



-1838 



1 : 11 -4 



3 



32 -80 



-0202 



-2898 



1 : 14 -6 



0-5 



60-08 



-0370 



-4630 



1 : 12 -5 



0-8 



108 -75 



0-0669 



-7331 



1 : 11 -0 



1-0 



130 -88 



-0806 



-9194 



1 : 11 -4 



2-0 



211 -71 



-1303 



1 -8697 



1 : 14 -3 



3-0 



245 -49 



-1511 



2 -8498 



1 : 18 -9 



4-0 



279 -28 



-1719 



3 -8281 



1 : 22 -3 



5 



306 -94 



-1889 



4 -8111 



1 : 25 -5 



