1905.] On certain Properties of Solutions of Chloroform, etc. 97 



At O'l per cent., complete solution, cloudy from proteid precipitation ; 

 0*2 per cent., all dissolved ; - 3 per cent., not all dissolved. 



In all cases the solubility in serum is higher than in water, and it is clear 

 from the results that a similar association between the anaesthetic and the 

 proteid of the serum occurs throughout the series. The amount of ethereal 

 extractive in the different samples of serum used for the determinations 

 varied between - 24 and - 36 per cent., thus showing that the increased 

 solubilities found could not be due to solution of the anaesthetic in lipoid or 

 ethereal extractives present in the serum. 



D. Changes in Depression of the Freezing Point of Water, Saline, and Serum 

 respectively caused by the addition of Chloroform. 



These experiments were undertaken to elucidate if possible the state in 

 which the chloroform existed in the proteid solutions. 



The determinations were made with Beckmann's apparatus. On account 

 of the chloroform being volatile, the apparatus had to be completely closed, 

 and the stirring accomplished by electro-magnet in the usual way. The 

 solution was placed in a straight tube, which was almost completely filled, so 

 that no correction was required for escape of chloroform into the air space 

 above the fluid. 



The changes in depression of freezing point are small, but in every case 

 the results are comparable and concordant. In the table (Table VI) the 

 percentages of chloroform are given by weight, and the figures are corrected 

 to allow for the degree of supercooling. 



Table VI. — Changes in A for Amounts of Chloroform added to Various 

 Solutions indicated at the Heads of the separate Columns. 



Distilled water 

 plus 0'6 per 



cent, 

 chloroform. 



Saline 

 solution 

 (0-75 per 

 cent.) plus 

 0'6 per cent, 

 chloroform. 



Serum 

 plus - 6 per 



cent, 

 chloroform. 



Serum 

 plus TO per 



cent, 

 chloroform. 



Serum 

 plus 

 2-0 per 

 cent, 

 chloroform. 



Serum 

 plus 

 3-0 per 

 cent, 

 chloroform. 



Serum 

 plus 

 4'0 per 

 cent, 

 chloroform. 



0-061 



0-081 



0-060 



0-101 



0-135 



0-138 



0-142 



0-075 



0-075 



0-062 



0-086 



0-141 







0-077 



0-072 



0-072 



0-104 









0-099 



0-082 













Average -078 



0-078 



0-065 



0-097 



0-138 



0-138 



0-142 



It is noticeable from the table (1) that the lowering of freezing point for 

 chloroform in serum is less than the lowering due to the same amount of 



