116 



Prof. Moore, Dr. Koaf, and Mr. Whitley. 



[Oct. 9, 



Table VI. — Calcium Hydrate. 



Experiment 1, Brood No. 5. The lime-water added required for neutrali- 

 sation of 50 c.c, in one case 25 - 25 c.c. of N/10 HC1, and in a second 

 trial 25*28 c.c. ; this lies so near 25 c.c. that the solution was taken 

 as N/20. 



No. 



Amount of 



calcium 

 hydrate in 

 N/20 solu- 

 tion, added 

 to 200 c.c. of 

 sea-water. 



Amount of 

 added alkali 

 in the 

 solution, 

 expressed as 



Observations of embryos at stated times after commencement 

 of experiment. 





a fraction 

 of normal 

 strength. 



18 hrs. 



42 hrs. 



1 



Control 





Well-developed blastulse 



No further development. Com- 

 mencing degeneration 



2 



1 c.c. 



-00025 



Blastulse clearly more ad- 

 vanced than control, and 

 showing more cell-division 



Late blastulse, one or two 

 moving 



3 



2 „ 



0-0005 





Same as No. 2, but more in 









motion 



4 



4 „ 



o-ooi 



Well-developed blastulse, but 

 some deformed in shape 



Blastulse, but motionless 



5 



6 „ 



-0015 



Morulae, and occasional small 

 blastulse 



Blastulse, but not so far 

 developed as No. 4 



6 



8 „ 



0-002 



Nearly all single-celled ; but 

 occasional two-, three- or 

 five-celled group 



Nearly all single-celled. Those 

 showing development are 

 chiefly early irregular morulsB. 

 Single cells show extended 

 layer. 



7 



10 „ 



0.0025 



Single cells in many cases 

 pear-shaped 



No development ; single cells 

 showing extended layer 



A second experiment gave similar results. 



