1905.] Kala-Azar, etc., from Leishman-Donovan Bodies. 



293 



several months of the cold season most closely correspond with that which 

 L have found to be most favourable to the development of the flagellated 

 stage of the Hepatomonas of kala-azar, namely Assam, Bengal, and Madras. 

 On the other hand, the disease is much rarer, or has not yet been proved to 

 originate, in those parts of India where the winter season presents a 

 greater degree of cold, and the more favourable spring and autumn are 

 very short. 



Much work will be necessary to test the truth or otherwise of the above 

 hypothesis, but knowledge should mean power to prevent the most terrible 

 of all tropical diseases in its combined very high mortality and slow death 

 by inches, and as the most favourable cold weather working season is 

 approaching, it appears to be advisable to put these observations on record 

 for the benefit of other workers in this very important field of tropical 

 medicine. • 



REFERENCES. 



I. Leishman, "On the Possibility of the Occurrence of Trypanosomiasis in India," 



'Brit. Med. Journ.,' vol. 1, 1903, p. 1252. 

 ± ' Brit. Med. Journ., 5 vol. 2, 1903, p. 79. 



3. ' Bull, de l'Acad. de Med.,' vol. 1, p. 238. 



4. < Lancet,' vol. 2, 1903, p. 44. 



5. 'Brit. Med. Journ.,' vol. 2, 1903, p. 1401. 



6. 'Scientific Memoirs, Government of India,' No. 18, New Series. 



7. ' Brit. Med. Journ.,' September, 1904. 



8. ' Quart. Journ. of Micro. Sci.,' November, 1904. 



9. 'Lancet,' vol. 1, 1905, p. 10. 



10. ' Scientific Memoirs, Government of India,' No. 15, New Series. 

 J 1. ' Journ. of Boy. Army Med. Corps,' March, 1905. 



12. 'Journal of Infectious Diseases,' vol. 2, 1905, p. 256. 



13. ' Scientific Memoirs, Government of India,' No. 11, New Series. 



DESCRIPTION OP PLATE. 

 Magnification of all the figures 1500 diameters. 

 Pig. I. — Undeveloped Leishman-Donovan bodies from spleen puncture film. 

 „ II. — Early stages of development, from two days' culture in acidified citrated blood ; 



1 and 2, body and macronucleus enlarged ; 3 and 4, first appearance of eosin 

 body ; 5 and 6, elongation and subdivision ; 7 and 8, first appearance of 

 flagelhim. 



., III. -Stages of division of the early flagellated forms. 

 IV. — Double long swimming forms. 

 V. — Fully developed long, free, active single cells. 

 ,, VI. — Degenerate forms. 



VII. — Undeveloped forms in a white corpuscle. 

 ,, VIII. — Early stages of development in a degenerating white corpuscle. 

 ., IX. — Stage in the formation of rosette. 

 „ N. — Separated flagella with micronuclei attached. 

 ,, NI. — Rosette breaking up into free forms. 

 ,, XII. — Small complete rosette. 



