412 



Dr. A. Harden and Mr. W. J. Young. 



[Dec. 8, 



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of the liquid, and not the diminution 

 of the amount of fermentation with 

 diminishing concentration of sugar, an 

 excess of this substance being present 

 throughout. 



A comparison of the two curves shows 

 very clearly the two factors involved 

 in the great increase in Experiment B : 



(1) The initial rapid evolution, and 



(2) the prolongation of the fermenta- 

 tion. 



5. The Initial Period of Rapid Evolution of Caroon Dioxide. 



This is a very striking phenomenon, and a typical example is illustrated in 

 fig. 2 in which the curves show the course of the evolution of carbon dioxide 

 (total volume evolved plotted against time) during two hours in the ease of: 



A. 25 c.c. yeast-juice +75 c.c. water +10 grammes glucose + toluene. 



B. 25 c.c. yeast-juice +50 c.c. water +25 c.c. boiled autolysed yeast-juice 

 + 10 grammes glucose + toluene. 



C. 25 c.c. yeast-juice +75 c.c. boiled autolysed yeast-juice +10 grammes 

 glucose + toluene. 



In B and C the initial rates are almost equal (58 c.c. in 10 minutes) and 

 much greater than in A (14 c.c. in 10 minutes). In B the rate rapidly falls 



