514 Dr. J. E. Lane-Claypon and Prof. E. H. Starling. [Feb. 12, 



placenta and membranes, or of the foetus alone. Finally, we made certain 

 control experiments in which the animals were injected either with emulsion 

 of liver or with blood serum obtained from normal animals. 



I. — Ovaries. 



Experiment 1. April 22, 1904. —Two ovaries from rabbit 15 days pregnant were 

 implanted into peritoneum of second non-pregnant rabbit. 



May 3. — Rabbit killed. No change in mammary glands ; the implanted ovaries were 

 necrosed and vascularised ; uterus and vulva congested. On microscopic examination 

 there was no proliferation of mammary gland. The uterine mucous membrane was typical 

 of " heat." 



The rabbit from which the ovaries were taken aborted immediately after the operation. 



Experiment 2. May 31, 1904. — A l'abbit which had been in the laboratory since 

 January was injected with chloroform water extract of six ovaries from three rabbits, 

 each about the fifteenth day of pregnancy. The rabbit received three injections in the 

 course of nine days. 



It was killed on June 13, four days after the last injection. No changes were found in 

 the mammary glands, and there were no changes in the uterus. 



Experiment 3. June 6, 1904. — A virgin rabbit was injected on six occasions during 

 19 days with an aseptic emulsion of 10 ovaries from rabbits between 11 and 14 days 

 pregnant. 



June 20. — Killed. Mammary glands invisible, uterus enlarged and congested, with 

 proliferation of mucous membrane. 



Experiment 4. May 16, 1904. Virgin rabbit received 13 injections of the saline extract 

 of 26 ovaries between May 16 and June 1. 



June 1. — Killed. The uterus slightly congested. No effect on mammary glands. 



IT. — Uterus and Uterine Mucous Membrane. 



Experiment 5. May 30, 1904. — Virgin rabbit, about six months old, injected with 

 chloroform water extract of the mucous membrane of four pregnant uteri. During 14 

 days it received four injections. 



It was killed on June 20. No changes, either of mammary glands or of uterus. 



Experiment 6. October 12, 1904. — Two virgin rabbits received during a period of 

 19 days seven injections of the filtered saline extract of the mucous membrane of seven 

 pregnant uteri. One rabbit (A) received the boiled extract, the other rabbit (B) received 

 the unboiled extract. Rabbit A was killed on October 31. The uterus and ovaries were 

 congested (the animal was in heat), but there was no growth of the mammary glands. 



Rabbit B, killed on November 2. The mammary glands showed no change, but the 

 uterus in this one was also congested. 



Two other experiments were made on the injection of uterine mucous membrane, but 

 with no result. 



III.— Injection of Placenta. 



Experiment 7. May 15, 1904. — A virgin rabbit received the unboiled extracts (press 

 juice) of the mucous membrane of 14 pregnant uteri and 123 placentae. These were given 

 in 14 injections between May 15 and June 1. It was killed on June 2. The mammary 

 glands were unaltered. The uterus was congested. 



Experiment 8. October 12, 1904. — Two virgin rabbits, A and B. 



A received on eight occasions during 19 days the boiled extract of 55 placentte. It was 

 killed on October 31. No results either on uterus or mammary glands. 



