1906.] Antityphoid Serum obtained from the Goat 551 

 II. — Tests of Serum of Goat I. 



Date. 



June 23 



July 7 



)> 



3J "" 



3J 



. . . . . 



July 14 



. . . . 



July 21 .... 



„ .... 

 jj .... 



,, .... 



» 



August 4 . 

 August 11. 



Test animal. 



Eabbit 1 

 „ 2 



» I 

 » 2 

 » 3 



5 



» 1 

 » 2 

 u 3 

 „ 4 



Amounts injected. 



3 e.c. serum + 1 c.o. toxin. 

 Control, 1 c.c. toxin. 



3 c.c. serum + 2 c.c. toxin. 

 3 „ +1 „ 



2 „ +1 „ 

 Control, 2 c.c. toxin. 



» 1/20 „ 



3 c.c. serum + 2 c.c. toxin. 

 2 „ +2 „ 



1 » +1 „ 

 1/10 „ +1 



Control, 1/10 c.c. toxin. 

 „ 1/20 „ 



1 c.c. serum + 1 c.c. toxin. 

 1/2 „ +1 „ 

 1/10 „ +1 



Control, 1 c.c. toxin. 



„ i/io 



» 1/15 



1/20 c.c. serum + 1 c.c. toxin. 

 1/50 „ +1 „ 

 1/100 „ +1 



Control not made. 



1/20 c.c. serum + 1 c.c. toxin. 

 1/50 „ +1 

 1/100 „ +1 



Control, 1 c.c. toxin. 

 „ 3/10 „ 



» i/io „ 



Eesidts. 



Alive. 

 Dead. 



Alive. 



Dead, 4 hours. 



18 " » 



Alive. 



Dead. 



Diarrhoea and collapse. 

 Alive. 



Dead. 



Diarrhoea and collapse. 



» )» 13 



Alive. 



Dead. 



2 hours. 

 18 „ 

 18 „ 



The death of the goat prevented further injections and tests being made. 

 :It will be seen that demonstrable antiendotoxins had developed in the goat 

 and that the results were encouraging. After 12 injections of typhoid cell 

 juice, 1/50 c.c. of the goat's serum protected a rabbit against 10 lethal doses 

 of the typhoid endotoxin. This property was not present in 3 c.c. of normal 

 goat's serum. It is not my intention in this paper to refer in detail to 

 the production of agglutinins and bacteriolysins. It will be sufficient 

 to state that whilst the amount of agglutinins present in the serum varied, 

 the highest titrate obtained was in a dilution of 1/1,000,000. After 

 12 injections of typhoid cell juice, 1/10000 c.c. of the goat's serum protected 

 guinea-pigs on intraperitoneal injection against 10 lethal doses of the 

 B. typhosus. The serum was, therefore, proved to possess marked anti- 

 endotoxic, agglutinative and bacteriolytic properties. 



The next step was to control these results by the immunisation of fresh 



