552 Dr. A. Macfadyen. Upon the Properties of an [Mar. 2, 



goats, and to carry out the process in a still more careful manner. The 

 method adopted is given in the following schedule : — 



Nanny Goat II— Eeceived following intravenous injections of toxic cell 

 juices of B. typhosus — 



1905. 1905. 



Oct. 13 



1/20 



c.c. 



111. 



Dec. 22 



.. 1/8 



c.c. 



No symptoms. 



„ 20 ... 



1/20 



n 



No symptoms. 



„ 29 ... 



.. 1/6 



» 



111. 



„ 27 .... 



,. 1/15 



j) 



111. 



1906. 









Nov. 3 



, 1/15 



n 



No symptoms. 





.. 1/3 





No symptoms. 



„ 10 .... 



1/10 



)> 



No symptoms. 



„ 12 .... 



.. 1/6 



» 



No symptoms. 



„ 17 .... 



1/10 



!> 



111. 



„ 19 .... 



.. 1/4 



ii 



No symptoms. 



„ 24 .... 



1/10 



11 



111. 



„ 26 .... 



.. 1/4 



ii 



No symptoms. 



Dec. 1 , 



1/10 



11 



No symptoms. 



Feb. 2 



1/3 



ii 



111. 



„ 8 .... 



.. 1/8 



11 



111. 



„ 9 .... 



.. 1/3 



ii 



No symptoms. 



„ 15 .... 



.. 1/8 



» 



No symptoms. 











Ill— Tests of Serum of Goat II. 



Date. 



Test animal. 



Amounts injected. 



Eesults. 



December 29 



January 19, 1906. 



January 26 



February 2 



February 9 



Babbit 1 



„ 2 



„ 3 



„ 4 



„ 5 



„ 1 



„ 2 



„ 3 



„ 1 



„ 2 



„ 3 



„ 1 



., 2 



„ 3 



„ 4 

 5 



„ 6 



» 7 



» 1 



« 2 



., 3 



» 4 



.. 5 



1/20 c.c. serum + 1 c.c. toxin. 

 1/50 „ +1 

 1/100 „ +1 



Control, 1/5 c.c. toxin. 



i/io 



1/50 c.c. serum + 1 c.c. toxin. 

 1/100 „ +1 



Control, 1/10 c.c. toxin. 



1/50 c.c. serum + 1 c.c. toxin. 

 1/100 „ +1 



Control, 1/10 c.c. toxin. 



1/10 c.c. serum + 1 c.c. toxin. 

 1/20 „ +1 

 1/50 „ +1 „ 

 1/50 „ +1/2 



Control, 1/10 c.c. toxin. 



„ 1/20 „ 



„ 1/30 „ 



1/50 c.c. serum + 1 c.c. toxin. 

 1/50 „ +1 

 1/100 „ +1 

 1/100 „ +1/2 



Control, 1/10 c.c. toxin. 

 „ 1/20 



Alive. 

 Dead. 

 Alive. 



Dead, 2 hours. 

 Diarrhoea and collapse. 



Alive. 

 Dead. 



Alive. 

 Dead. 



Alive. 



Dead, 2£ hours. 

 „ 2 „ 

 „ 18 „ 



Alive. 



Dead. 

 Alive. 

 Dead. 



Diarrhoea and collapse. 



Three goats are in process of immunisation by this method of small and 

 gentle dosage and have not up to the present succumbed. It will be 

 observed that whilst in the case of Goat I the dosage was raised from 



